Chemical constituents of Stelmatocrypton khasianum and their biological activity
10.3969/j.issn.1001-1528.2025.11.017
- VernacularTitle:生藤化学成分及其生物活性研究
- Author:
Jing YU
1
;
Guo-xu MA
;
Cui-yun YIN
;
Zhao-you DENG
;
Yi-hang LI
;
Er LI
Author Information
1. 中国医学科学院药用植物研究所云南分所,云南省南药可持续利用研究重点实验室,云南景洪 666100
- Publication Type:Journal Article
- Keywords:
Stelmatocrypton khasianum(Benth.)Baill.;
chemical constituents;
isolation and identification;
anti-inflammatory activity;
cytotoxic activity
- From:
Chinese Traditional Patent Medicine
2025;47(11):3645-3650
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Stelmatocrypton khasianum.(Benth.)Baill.and their in vitro anti-inflammatory activity and cytotoxic activity.METHODS Separation and purification were performed using thin layer chromatography,silica gel,semi-preparative HPLC and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro anti-inflammatory activity was evaluated by RAW264.7 model,and the cytotoxic activity was determined by CCK-8 method.RESULTS Fifteen compounds were isolated and identified as 2α,3β,19α,23-tetrahydroxy-urs-12-en-28-oic acid-3-O-β-D-glucopyranosyl-28-O-β-D-glucopyranosyl ester(1),dalzienoside(2),benzyl-(6-O-α-L-rhanmopyranosyl)O-β-D-glucopyranoside(3),corchorusoside C(4),cyclo(Ala-Tyr)(5),thymidine(6),(4S,5S)-5-hydroxy-4-hexanolide(7),2-methylpyridin-3-ol(8),butyl isobutyl phthalate(9),bis-(2-ethylhexyl)terephthalate(10),p-hydroxybenzaldehyde(11),vanilic acid(12),salicylic acid(13),isovanilic acid(14),3-hydroxy-p-anisaldehyde(15).The IC50 values of compounds 1,2 and 4 for NO were(27.69±5.51),(25.82±3.58)and(23.35±7.09)μmol/L,respectively.The IC50 value of compound 1 on MCF-7 cells was(18.15±6.45)μmol/L.The IC50 values of compound 4 on MCF-7 and HCCC-9810 cells were(19.43±2.66)and(21.76±5.81)μmol/L,respectively.CONCLUSION Compounds 2-11 are isolated from S.khasianum for the first time.Compounds 1,2 and 4 exhibit good in vitro anti-inflammatory activity,and 1,4 have cytotoxic activity.
