Effects of Qinhuo Formula on inflammation and TLR4/MyD88/NF-κB signaling pathway in obese mice induced by high-fat diet
10.3969/j.issn.1001-1528.2025.11.011
- VernacularTitle:芩藿方对高脂饮食诱导的肥胖小鼠炎症及TLR4/MyD88/NF-κB信号通路的影响
- Author:
Yao-hua LEI
1
;
Jing WANG
;
Shan LIU
;
Yuan TIAN
;
Xiao-liang LI
;
Xin-ying ZHUANG
Author Information
1. 云南中医药大学中药学院暨云南省南药可持续利用研究重点实验室,云南 昆明 650500
- Publication Type:Journal Article
- Keywords:
Qinhuo Formula;
obesity;
glucolipid metabolism;
inflammatory response;
TLR4/MyD88/NF-κB signaling pathway
- From:
Chinese Traditional Patent Medicine
2025;47(11):3607-3613
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the improvement effect of Qinhuo Formula on obese mice induced by high-fat diet.METHODS Obese mice model was induced by feeding with high-fat diet.The mice successfully established were randomly divided into model group,orlistat group(15.6 mg/kg)and low and high dose groups of Qinhuo Formula(13.5 and 22.5 g/kg),and normal mice were taken as control group,with 10 mice in each group,and the drugs were continuously intervened for 8 weeks.After the drug administration,the body weight,abdominal circumference,fasting blood glucose and glucose tolerance were measured,and area under the curve(AUC)of oral glucose tolerance test(OGTT),Lee's index and fat index were calculated.The serum levels of TC,TG,LDL-C and HDL-C were detected by blood biochemical analyzer.Serum levels of TNF-α,IL-1 β and IL-6 were detected by ELISA.HE staining was used to observe the white fat of epididymis and the pathological changes of liver tissue.RT-qPCR and Western blot were used to detect the mRNA and protein expressions of TLR4,MyD88 and NF-κB in liver tissue.RESULTS Compared with the model group,the body weight,abdominal circumference and Lee's index of mice in each dose group of Qinhuo Formula decreased(P＜0.01);OGTT-AUC decreased(P＜0.01);visceral fat index decreased(P＜0.05,P＜0.01);the levels of serum TG,TC,IL-6 and IL-1 β decreased(P＜0.01);the diameter of epididymal adipocytes decreased,arranged tightly and regularly,the lipid droplet cavities in liver tissue decreased,and the morphology of hepatocytes recovered;the mRNA and protein expressions of TLR4,MyD88 and NF-κB decreased(P＜0.05,P＜0.01).CONCLUSION Qinhuo Formula can effectively improve glucose-lipid metabolism and reduce inflammation in obese mice,and its mechanism may be related to the inhibition of TLR4/MyD88/NF-κB signaling pathway.
