Optimization of cellulase-assisted ultrasound extraction process for total flavonoids from Plumbago zeylanica and evaluation of their anti-oxidant activity
10.3969/j.issn.1001-1528.2025.11.007
- VernacularTitle:白花丹总黄酮纤维素酶辅助超声提取工艺优化及其抗氧化活性评价
- Author:
Xiao-lu GAO
1
;
Wen-de CHENG
;
Yue-yuan WEN
;
Shang-ping XING
;
Cheng SHI
;
Dan ZHU
;
Ya-nan XU
Author Information
1. 广西医科大学药学院,广西南宁 530021
- Publication Type:Journal Article
- Keywords:
Plumbago zeylanica L.;
total flavonoids;
cellulase;
ultrasound extraction process;
anti-oxidant activity;
response surface method
- From:
Chinese Traditional Patent Medicine
2025;47(11):3580-3585
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the cellulase-assisted ultrasound extraction process for total flavonoids from Plumbago zeylanica L.,and to evaluate their anti-oxidant activity.METHODS With extraction time,liquid-solid ratio,cellulase addition amount,extraction temperature and ultrasonic power as influencing factors,extraction rate of total flavonoids as an evaluation index,the extraction process was optimized by response surface method on the basis of single factor test.Subsequently,The scavenging rates of extract on DPPH,ABTS and OH free radicals were determined.RESULTS The optimal conditions were determined to be 34∶1 for liquid-solid ratio,3％for cellulase addition amount,51 ℃ for extraction temperature,38 min for extraction time,and 400 W for ultrasonic power,the extraction rate of total flavonoids was(33.411±0.97)％.The IC50 values of three free radicals were 0.13,0.042,3.29 mg/mL,respectively.CONCLUSION This reasonable and reliable method can be used for the cellulase-assisted ultrasound extraction of total flavonoids from P.zeylanica with strong anti-oxidant activity.
