Quality improvement of Compound Yuxingcao Tablets based on HPLC fingerprints and content determination
10.3969/j.issn.1001-1528.2025.11.003
- VernacularTitle:基于HPLC指纹图谱及含量测定完善复方鱼腥草片质量
- Author:
Guang-li AI
1
;
Xin WANG
;
Ji LI
;
Ting-ting LI
;
Xiao LUO
;
Shuang-cheng MA
Author Information
1. 成都市药品检验研究院,四川成都 610045;国家药品监督管理局中药材质量监测评价重点实验室,四川成都 610045
- Publication Type:Journal Article
- Keywords:
Compound Yuxingcao Tablets;
quality improvement;
HPLC fingerprints;
content determination;
principal component analysis;
orthogonal partial least squares discriminant analysis
- From:
Chinese Traditional Patent Medicine
2025;47(11):3548-3554
- CountryChina
- Language:Chinese
-
Abstract:
AIM To improve the quality of Compound Yuxingcao Tablets.METHODS The HPLC fingerprints were established,after which the contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,forsythoside I,forsythoside A,quercitrin,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid,baicalin,oroxyloside,wogonin and baicalein were determined.The analysis was performed on a 30 ℃ thermostatic Waters XBridge C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 327 nm.Subsequently,principal component analysis and orthogonal partial least squares discriminant analysis were adopted.RESULTS There were 21 common peaks in the fingerprints for 22 batches of samples with the similarities of more than 0.85.Twelve constituents showed good linear relationships within their own ranges(R20.999 8),whose average recoveries were 93.68％-101.16％with the RSDs of 0.95％-2.35％.Baicalin,wogonin and forsythoside A were taken as quality differential components.CONCLUSION This simple and accurate method can be used for the quality control of Compound Yuxingcao Tablets.
