Simultaneous content determination of twenty-two saponins in Dengzhan Shengmai Capsules by UPLC-MS/MS
10.3969/j.issn.1001-1528.2025.11.001
- VernacularTitle:UPLC-MS/MS法同时测定灯盏生脉胶囊中22种皂苷的含量
- Author:
Shan-shan ZHUANG
1
;
Lin ZHOU
;
Fang HONG
;
Long LIN
;
Si-rong LIN
;
Ming-qing HUANG
Author Information
1. 福建省食品药品质量检验研究院,福建 福州 350012
- Publication Type:Journal Article
- Keywords:
Dengzhan Shengmai Capsules;
saponins;
content determination;
UPLC-MS/MS;
cluster analysis;
principal component analysis;
partial least squares discriminant analysis
- From:
Chinese Traditional Patent Medicine
2025;47(11):3533-3540
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneouscontent determination of notoginsenosides R1,Fc,Fe,ginsenosides Re,Rg1,Rf,F3,Rg2,Ra2,Rb1,Ro,Rc,F1,Ra1,Rb2,Rb3,Rd,F2,Rg5,chikusetsusaponin Ⅳ a,20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 in Dengzhan Shengmai Capsules.METHODS The analysis was performed on a 35 ℃ thermostatic Accucore Phenyl Hexyl column(2.1 mm×100 mm,2.6 μm),with the mobile phase comprising of 0.1％formic acid-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and heated electrospray ionization source was adopted in negative ion scanning with parallel reaction monitoring mode.Subsequently,cluster analysis,principal component analysis and partial least squares discriminant analysis were adopted.RESULTS Twenty-two saponins showed good linear relationships within their own ranges(r ≥ 0.996 4),whose average recoveries were 98.5％-101.6％with the RSDs of 1.3％-5.2％.Ten batches of samples were clustered into four types,three principal components demonstrated the accumulative variance contribution rate of 90.265％,ginsenosides Rb1,Rg2,Rb2,Rd,Rg1,Ro,Rf,Re,Rg5,Rc were taken as potential quality markers.CONCLUSION This simple,efficient,accurate and sensitive method can be used for the quality control of Dengzhan Shengmai Capsules.
