Effects of Dihong Prescription Regulating Mitochondrial Function on Glomerular Mesangial Cells Cultured with High Glucose
10.19879/j.cnki.1005-5304.202502458
- VernacularTitle:地红方含药血清调控线粒体功能对高糖培养肾小球系膜细胞的影响
- Author:
Zi LIN
1
;
Weidong HE
;
Sizhe DU
;
Xiaoyu LIN
Author Information
1. 福建中医药大学附属人民医院,福建 福州 350004
- Publication Type:Journal Article
- Keywords:
Dihong Prescription;
diabetic nephropathy;
oxidative stress;
apoptosis;
mitochondrial fission;
mitophagy;
glomerular mesangial cells
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(9):69-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Dihong Prescription on glomerular mesangial cells of mice cultured with high glucose;To explore its mechanism in the treatment of diabetic nephropathy.Methods The TargetScan database was used to predict the miRNA regulating bispecific phosphatase 1(DUSP1),and the double luciferase was used to verify the targeting relationship between miR-133b and DUSP1.The glomerular mesangial cells of mice were cultured in vitro and divided into control group,high glucose group and Dihong Prescription low-,medium-and high-dosage groups,and was given corresponding intervention.CCK-8 was used to detect cell viability,TUNEL staining was used to observe the apoptosis of cells,flow cytometry was used to determine the expression of reactive oxygen species(ROS),Western blot was used to detect the protein expressions of phosphorylated extracellular regulatory protein kinase 1/2(p-ERK1/2),ERK1/2,phosphorylated c-Jun amino-terminal kinase(p-JNK),JNK,p-p38,p38,mitochondrial dynamoculture-associated protein 1(Drp1)and mitochondrial fission protein 1(Fis1),transmission electron microscopy was used to observe the ultrastructure of mitochondria.Results The double luciferase demonstrated that miR-133b could target the regulation of DUSP1.Compared with the control group,the cell viability significantly decreased in the high glucose group(P<0.01),the apoptosis rate significantly increased(P<0.01),the content of ROS significantly increased(P<0.01),and the protein expressions of p-ERK1/2/ERK1/2,p-p38/p38,p-JNK/JNK,Drp1 and Fis1 significantly increased(P<0.01).Compared with the high glucose group,the cell viability significantly increased in Dihong Prescription medium-and high-dosage groups(P<0.01),the apoptosis rate significantly decreased(P<0.01),the content of ROS significantly decreased(P<0.01),and the protein expressions of p-ERK1/2/ERK1/2,p-p38/p38,p-JNK/JNK,Drp1 and Fis1 significantly decreased(P<0.01).Transmission electron microscopy showed that large number of autophagosomes,autophagosomes,mitochondria vacuolation and cristae fusion were observed in the high glucose group;the mitochondrial damage caused by high glucose was alleviated in Dihong Prescription low-,medium-and high-dosage groups.Conclusion Dihong Prescription can improve oxidative stress injury in glomerular mesangial cells of mice cultured with high glucose,and its mechanism may be related to the regulation of mitochondrial function and miR-133b/DUSP1/MAPK pathway.
