Clinical characteristics of 9 cases of Talaromyces marneffei infection and its early rapid diagnosis using third-generation nanopore sequencing technology
10.11816/cn.ni.2025-246755
- VernacularTitle:9例马尔尼菲篮状菌感染临床特征及其三代纳米孔测序技术早期快速诊断
- Author:
Chunyan ZHAO
1
;
Chang SONG
1
;
Aichun HUANG
1
;
Chaoyan XU
1
;
Xiaoying WEI
1
;
Chunmei ZENG
1
;
Qingdong ZHU
1
Author Information
1. 南宁市第四人民医院结核科,广西 南宁 530023
- Publication Type:Journal Article
- Keywords:
Talaromyces marneffei infection;
Nanopore sequencing;
Rapid diagnosis;
Imaging findings;
Treatment prognosis
- From:
Chinese Journal of Nosocomiology
2025;35(15):2288-2292
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the potential of nanopore sequencing technology for rapid diagnosis of Talaro-myces marneffei(TM)infection.METHODS Nine patients with TM infection admitted to the Fourth People's Hospital of Nanning from May 13,2022 to Aug.3,2023 were retrospectively analyzed.Rapid diagnosis was con-ducted by nanopore sequencing technology,and a comprehensive analysis of their clinical characteristics and treat-ment processes was performed.RESULTS The 9 patients included in the study had infections in various sites such as the lungs,buttocks,blood and cervical lymph nodes.Comorbidities included AIDS,secondary pulmonary tuberculosis,non-tuberculous mycobacterial disease and adult-onset immune deficiency.Patients generally exhibited elevated C-reactive protein levels and erythrocyte sedimentation rates,along with increased neutrophil counts.Some patients had abnormal lymphocyte counts and CD4+/CD8+ratios.Microbiological tests showed positive cultures in 3 cases,positive smears in 2 cases and positive targeted detection in 6 cases.Nanopore sequencing detected various pathogens in the 9 patients.The treatment results indicated that 8 patients improved after medication,with 6 patients having medication regimens adjusted based on nanopore sequencing results.CONCLUSION Nanopore sequencing technology has shown potentials in the auxiliary diagnosis of TM infection,providing timely etiological diagnostic evidence for clinical practice.
