Identification methods of a strain of Anaerobiospirillum succiniciproducens
10.11816/cn.ni.2025-246879
- VernacularTitle:1株产琥珀酸厌氧螺菌鉴定方法
- Author:
Hua WU
1
;
Pei ZHANG
;
Pinghua QU
;
Yan WANG
;
Fenghua WU
;
Yujie JIANG
Author Information
1. 海南省人民医院,海南医科大学附属海南医院检验科,海南 海口 570100
- Publication Type:Journal Article
- Keywords:
Anaerobiospirillum;
Anaerobiospirillum succiniciproducens;
16sRNA;
Biochemical identification;
MALDI-TOF MS
- From:
Chinese Journal of Nosocomiology
2025;35(15):2253-2257
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide scientific and reasonable recommendations for the identification of Anaerobio-spirillum succiniciproducens in clinical microbiology laboratories by comparing the effectiveness of biochemical methods,matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),and 16S rRNA gene sequencing.METHODS Methodological evaluation was conducted.A strain of A.succiniciprodu-cens was isolated on Mar.27,2023 from a patient with sepsis caused by a dog bite,who was admitted to Guang-dong Nongken Central Hospital,and analyzed for the morphological characteristics,culture methods,and colony features.The identification of the isolated strain was conducted by the automated microbial identification and anal-ysis system(VITEK 2 Compact 2,bioMerieux),MALDI-TOF MS including VITEK MS,MALDI Biotyper,Au-tof ms 1000,and EX-Accuspec,as well as 16S ribosomal RNA(rRNA)gene sequencing.The performance of each method was evaluated.RESULTSA.succiniciproducens was a strictly anaerobic gram-negative spiral bacteri-um,characterized by slow growth,flat and colorless transparent colonies,and negative oxidase and catalase activ-ity.When using VITEK 2 Compact ANC card,it was not successfully recognized;however,through the identifi-cation by VITEK MS,MALDI Biotyper,Autof ms 1000 and EX-Accuspec,it was all confirmed as A.succinicip-roducens.Additionally,16S rRNA gene sequencing technology also identified it as A.succiniciproducens.CONCLUSIONS Current automated biochemical identification systems are unable to accurately identify A.succi-niciproducens.MALDI-TOF MS and 16S rRNA gene sequencing techniques can reliably confirm A.succinicipro-ducens.Clinical microbiologists should select the appropriate identification method based on their available re-sources.
