Bactericidal effect of levulinic acid-sodium dodecyl sulfate against Mycobacterium abscessus,Mycobacterium chelonae and Mycobacterium fortuitum
10.11816/cn.ni.2025-246948
- VernacularTitle:乙酰丙酸-十二烷基硫酸钠对脓肿分枝杆菌与龟分枝杆菌和偶发分枝杆菌的杀灭作用
- Author:
Saisai WANG
1
;
Qi SUN
;
Hongyan JIA
;
Liping PAN
;
Yang LIU
Author Information
1. 首都医科大学附属北京胸科医院疾病预防控制处/北京市结核病胸部肿瘤研究所,北京 101149
- Publication Type:Journal Article
- Keywords:
Levulinic acid plus sodium dodecyl sulfate;
Rapidly growing mycobacteria;
Mycobacterium abscessus;
Mycobacterium chelonae;
Mycobacterium fortuitum;
Disinfection efficacy
- From:
Chinese Journal of Nosocomiology
2025;35(15):2241-2247
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the bactericidal effect of levulinic acid-sodium dodecyl sulfate(LVA-SDS)on the strains of Mycobacterium abscessus,Mycobacterium chelonae and Mycobacterium fortuitum,and to provide a new method for the prevention of common pathogenic rapidly growing non-tuberculous mycobacteria(NTM)dis-ease.METHODS The minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)of LVA-SDS were detected by microculture method and suspension quantitative bactericidal test,respectively.Using 1000 mg/L chlorine-containing disinfectant(sodium hypochlorite)as the control,the killing effect of LVA-SDS at different times and concentrations on M.abscessus,M.chelonae and M.fortuitum were detected.RESULTS The MIC values of LVA-SDS against M.abscessus,M.chelonae and M.fortuitum were 0.0625%LVA+0.00625%SDS,0.03125%LVA+0.003125%SDS and 0.125%LVA+0.0125%SDS,respectively.The MBC values were 0.25%LVA+0.025%SDS,0.25%LVA+0.025%SDS,and 1%LVA+0.1%SDS,repectively.Within a cer-tain range,with the increase in LVA-SDS concentration and the extension of time(1-30 min),the bactericidal effect on M.abscessus,M.chelonae and M.fortuitum gradually enhanced.The killing rates of the 18%LVA+1.8%SDS for 5 minutes and the 6%LVA+0.6%SDS for 20 minutes to M.abscessus reached 100.00%;the kill-ing rates of the 18%LVA+1.8%SDS for 10 minutes and the 10%LVA+1%SDS for 25 minutes to M.chelo-nae reached 100.00%;while the killing rates of the 18%LVA+1.8%SDS for 5 minutes and the 6%LVA+0.6%SDS for 30 minutes to M.fortuitum reached 100.00%.Compared with 1000 mg/L chlorine-containing disin-fectant(sodium hypochlorite),LVA-SDS has equivalent bactericidal effects against M.abscessus and M.chelo-nae,and a better bactericidal effect against M.fortuitum.CONCLUSION LVA-SDS has a good bactericidal effect on M.abscessus,M.chelonae and M.fortuitum,and may be used in the disinfection of NTM in the future.
