Effects of Wenfei Jiangzhuo Formula on mitochondrial function of Aβ25-35-induced BV-2 cells based on PGAM5-Drp1 axis
10.3969/j.issn.1001-1528.2025.08.011
- VernacularTitle:基于PGAM5-Drp1轴探讨温肺降浊方对Aβ25-35诱导BV-2细胞线粒体功能的影响
- Author:
Ding ZHANG
1
;
Zhi-han HU
;
Ke-qing ZHOU
;
Wei CHEN
;
Hong-ling QING
;
Jun-jun XIANG
;
Yue-qiang HU
Author Information
1. 广西中医药大学研究生院,广西南宁 530001
- Publication Type:Journal Article
- Keywords:
Wenfei Jiangzhuo Formula;
Alzheimer's disease;
Aβ25-35;
PGAM-Drp1 axis;
mitochondrial homeostatic balance;
BV-2 cells
- From:
Chinese Traditional Patent Medicine
2025;47(8):2558-2565
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of Wenfei Jiangzhuo Formula on mitochondrial function of Aβ25-35-induced BV-2 cells.METHODS In the establishment of cell model of Alzheimer's disease(AD)using Aβ25-35 on the BV-2 cells,the optimal concentration and time point of Aβ25-35 intervention were determined;and the groups for the intervention of LFHP-1c group(inhibitor)or the serum containing Wenfei Jiangzhuo Formula were set up.The detection of the optimal intervention concentration and time point by CCK-8 assay;the observation of cell migration and apoptosis by Transwell assay and Hoechst 33342 staining;the detection of the positive expressions of PGAM5 and Drp1 by immunofluorescence;and the detection of cellular PGAM5,Drp1,OPA1,and Mfn1/2 mRNA and protein expressions by RT-qPCR and Western blot were conducted.RESULTS The best AD cell model was established by 48 h exposure to 5 μmol/L of Aβ25-35,and most active cell viability was achieved with the 48 h use of serum containing 20％Wenfei Jiangzhuo Formula.Compared with the control group,the model group displayed decreased number of cell migration,more bright blue positive apoptotic cells,increased number of PGAM5 and Drp1 positive cells and their mRNA and protein expressions(P＜0.05);and decreased mRNA and protein expressions of OPA1 and Mfn1/2(P＜0.05).Compared with the model group,the groups intervened with the medicines shared increased number of cell migration,less bright blue positive apoptotic cells,decreased number of PGAM5 and Drp1 positive cells and their mRNA and protein expressions(P＜0.05);and elevated OPA1 and Mfn1/2 mRNA and protein expressions(P＜0.05).CONCLUSION Wenfei Jiangzhuo Formula exerts cerebroprotective effects to improve cognition by reducing cell damage and improving the balance of mitochondrial homeostasis through PGAM5-Drp1 axis in AD model.
