Differences between boiled powder and decoction of Ermiao Powder based on volatile constituents and anti-rheumatoid arthritis activity
10.3969/j.issn.1001-1528.2025.08.005
- VernacularTitle:基于挥发性成分和抗类风湿关节炎活性考察二妙散煮散、饮片汤剂差异
- Author:
Yu-meng ZHANG
1
;
Hai-zhu JIN
;
Wen-yue JIA
;
Yang LIU
;
Hui-qin HAO
Author Information
1. 山西中医药大学,山西晋中 030619;山西省中医药交叉创新工程研究中心,山西晋中 030619
- Publication Type:Journal Article
- Keywords:
Ermiao Powder;
boiled powder;
decoction;
volatile constituents;
anti-rheumatoid arthritis activity;
GC-MS
- From:
Chinese Traditional Patent Medicine
2025;47(8):2514-2522
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the differences between boiled powder and decoction of Ermiao Powder.METHODS GC-MS was used to identify volatile constituents,after which the content determination of linalool,4-terpineol,α-terpineol,β-eucalyptol and taxifolin in distillate was performed,evaporation rate curve was drawn.The rat model for collagen-induced arthritis was established,then HE and SO/FG staining were conducted,and body weight,footpad swelling degree,arthritis score,immune organ(spleen,thymus)indices,serum inflammatory factors(IL-10,IL-6),ankle joint structure(foot claw swelling,micro-CT)and locomotor ability were detected.RESULTS Total 43 and 26 volatile constituents were identified in boiled powder and decoction,respectively.With the extension of boiling time,the average evaporation rates of 5 volatile constituents in the boiled powder distillate demonstrated the trends of first increase and then decrease,which reached a maximum at 15-20 min;those in the decoction distillate displayed the trends of decrease,which were not detected after 15 min except for β-eucalyptol.Compared with the decoction,the boiled powder exhibited stronger effects on improving foot swelling and arthritis score,alleviating pathological changes in joint tissues,inhibiting inflammatory factors and restoring motor ability.CONCLUSION More volatile constituents are observable in the boiled powder of Ermiao Powder than those in its decoction,along with stronger anti-rheumatoid arthritis activity.The optimal decocting endpoint is determined to be within 15 min in boiling water.
