Bidirectional Mendelian randomization and mediation analysis of the relationship between gut microbiota,metabolites,and colorectal cancer
10.3969/j.issn.1673-9701.2025.24.012
- VernacularTitle:肠道菌群、代谢物与结直肠癌的双向孟德尔随机化及中介分析研究
- Author:
Jiawei WANG
1
;
Zhonghua HONG
1
;
Hezhai YIN
1
Author Information
1. 嘉兴市中医医院肛肠科,浙江嘉兴 314001
- Publication Type:Journal Article
- Keywords:
Gut microbiota;
Metabolites;
Mendelian randomization;
Colorectal cancer
- From:
China Modern Doctor
2025;63(24):55-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the causal relationship between gut microbiota and colorectal cancer,and to investigate whether metabolites have a mediating effect through Mendelian randomization(MR)analysis.Methods Genome-wide association studies data of 473 gut microbiota,233 metabolites,and colorectal cancer were obtained from public databases.Bidirectional MR analyses were performed between gut microbiota,metabolites,and colorectal cancer,respectively.Subsequently,a two-step MR was used to explore whether specific gut microbiota affect the occurrence and development of colorectal cancer through metabolites.Results The study found that the abundances of 7 gut microbiota were positively correlated with the risk of colorectal cancer:Alloprevotella,Atopobiaceae,Clostridium S felsineum,Hungatella,Microvirga,Peptococcia and Ruminococcus A;The abundances of 4 gut microbiota were negatively correlated with the risk of colorectal cancer:Paenibacillales,Prevotella buccae,Psychroserpens and Succinivibrionaceae.Through the two-step mediation analysis,it was confirmed that the ratio of Triglycerides to total lipids ratio in medium very low-density lipoprotein could reduce the impact of Clostridium S felsineum as a risk factor for the occurrence of colorectal cancer.Conclusion The results of this study confirm the causal relationship between some gut microbiota,metabolites,and colorectal cancer,and discover potential interaction chains,providing ideas for the pathogenesis and prevention of colorectal cancer.
