Effect of miR-204-5p targeted regulation of TRIB3 on the proliferation of hippocampal neural stem cells in rats after hypoxic-ischemic brain damage
10.3969/j.issn.1673-9701.2025.23.001
- VernacularTitle:miR-204-5p靶向调节TRIB3对大鼠缺氧缺血性脑损伤后海马神经干细胞增殖的影响
- Author:
Ling FAN
1
;
Mengzao HE
1
;
Jinping JIANG
1
Author Information
1. 杭州市妇产科医院新生儿科,浙江 杭州 310008
- Publication Type:Journal Article
- Keywords:
miR-204-5p;
Tribbles homolog;
Hypoxic-ischemic brain damage;
Neural stem cells
- From:
China Modern Doctor
2025;63(23):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA-204-5p(miR-204-5p)on the proliferation of hippocampal neural stem cells in rats after hypoxic-ischemic brain injury(HIBD),and to analyze its relationship with Tribbles homolog 3(TRIB3).Methods In vitro primary hippocampal neural stem cell culture was conducted.The untreated cells were designated as the CK group,while those subjected to oxygen and glucose deprivation(OGD)injury treatment were classified as the OGD group.Subgroups were established based on OGD conditions:miR-NC group,miR-204-5p mimic group,silencing-NC group,silencing-TRIB3 group,miR-204-5p mimic+pcDNA3.1 group,and miR-204-5p mimic+pcDNA3.1-TRIB3 group.The expression of miR-204-5p and TRIB3 mRNA and the proliferation of hippocampal neural stem cells were detected.The target relationship between miR-204-5p and TRIB3 was analyzed.Results Compared with CK group,OGD group showed decreased expression of miR-204-5p,A value,proliferating cell nuclear antigen(PCNA)and CyclinD1 protein expression,and increased expression of TRIB3(P＜0.05).Compared with miR-204-5p mimic group,OGD group and miR-NC group showed increased expression of miR-204-5p,A value,PCNA and CyclinD1 protein,and decreased TRIB3 expression(P＜0.05).Compared with miR-204-5p mimic+pcDNA3.1-TRIB3 group,miR-204-5p mimic group and miR-204-5p mimic+pcDNA3.1 group showed increased TRIB3 expression and decreased A value,PCNA and CyclinD1 protein expression(P＜0.05).There were binding sites for miR-204-5p and TRIB3.Conclusion The overexpression of miR-204-5p can inhibit the expression of TRIB3 and promote the proliferation of hippocampal neural stem cells after HIBD.
