Study of growth differentiation factor 15 attenuated high glucose-induced apoptosis in mouse pancreatic islet βMIN6 cells
10.3969/j.issn.1673-9701.2025.23.008
- VernacularTitle:生长分化因子15减轻高糖诱导小鼠胰岛β细胞MIN6凋亡的研究
- Author:
Huan LI
1
;
Mengqi WU
;
Zhiqiang WEN
;
Huafei DENG
;
Shunrong LUO
Author Information
1. 湘南学院基础医学院病理生理学教研室,湖南郴州 423000
- Publication Type:Journal Article
- Keywords:
High glucose;
MIN6 cells;
Growth differentiation factor 15;
βcell
- From:
China Modern Doctor
2025;63(23):32-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of growth differentiation factor 15(GDF15)on apoptosis and insulin secretion of MIN6 cells under high glucose conditions.Methods The mouse pancreatic islet β cell line(MIN6 cells)were divided into four groups:NG group,NG+rGDF15 group,HG group,and HG+rGDF15 group.The cell morphology among groups were observed,the apoptosis rate,the protein expression levels of Bcl-2 and Bax and the insulin level was detected.Results HG group exhibited significant cellular damage,characterized by upregulated apoptosis-promoting protein Bax and downregulated apoptosis-suppressing protein Bcl-2 expression,accompanied by a marked increase in apoptosis rate and a substantial decrease in insulin secretion(P＜0.01).Administration of recombinant GDF15 protein improved MIN6 cell morphology,significantly reduced Bax protein relative expression,elevated Bcl-2 protein relative expression,markedly decreased apoptosis rate,and enhanced insulin secretion(P＜0.01).Conclusion GDF15 can mitigate high glucose-induced MIN6 cell damage.
