Phase-contrast CT for three-dimensional visualization of in vitro intrahepatic vessels and necrotic foci in mouse model of acute liver injury
10.13929/j.issn.1003-3289.2025.10.008
- VernacularTitle:相位对比CT技术用于三维可视化急性肝损伤小鼠模型离体肝内脉管及坏死灶
- Author:
Zibo WANG
1
;
Xianqin DU
;
Xiaohong XIN
;
Xinyan ZHAO
;
Chunhong HU
;
Wenjuan LYU
Author Information
1. 天津市胸科医院设备科,天津 300222
- Publication Type:Journal Article
- Keywords:
mice;
liver diseases;
necrosis;
phase-contrast computed tomography
- From:
Chinese Journal of Medical Imaging Technology
2025;41(10):1648-1652
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of phase-contrast CT for three-dimensional visualization of in vitro intrahepatic vessels and necrotic foci in mouse model of acute liver injury(ALI).Methods Six clean grade male wild-type C57BL/6 mice were randomly divided into model group and control group(each n=3).The mice in model group were fed with overdose of acetaminophen to induce ALI,while those in control group were fed with the same amount of distilled water.After 24 h,the mice were all sacrificed,the livers were harvested and then fixed and dehydrated.Phase-contrast CT images of in vitro liver were acquired,sectional and three-dimensional images were reconstructed.The effect of phase-contrast CT for displaying the outline of liver and internal vessels and necrotic foci were observed,the maximum diameter and volume of necrotic foci were quantitatively analyzed,and the results of phase-contrast CT were compared with pathological findings.Results The original projection and sectional images of phase-contrast CT clearly showed the outline of in vitro liver and internal vasculatures.Necrotic foci within the liver were found in model group,but not in control group.The findings of phase-contrast CT corresponded accurately to those of pathology.Three-dimensional reconstruction images of phase-contrast CT clearly displayed intrahepatic portal vein system and hepatic vein system in both groups,and discontinuous punctate necrotic foci within the liver were found in model group,mainly distributed around hepatic vein,with a median maximum diameter of 18.50 μm and a median volume of 5 870.11 μm3,but was not observed in control group.Conclusion Phase-contrast CT could be used in three-dimensional visualization of intrahepatic vessels and necrotic foci of in vitro liver in mouse model of ALI.
