Clinical correlation of P2X7R and NLRP3 inflammasomes in prostatic hyperplasia tissue
10.13263/j.cnki.nja.2025.11.004
- VernacularTitle:P2X7R与NLRP3炎性小体在良性前列腺增生组织中的表达及临床相关性分析
- Author:
Xuanzhen ZHOU
1
;
Chen HUANG
;
Changying MEI
;
Yingzhi CHEN
;
Zixiang LIU
;
Weiqi YIN
;
Zejun YAN
Author Information
1. 宁波大学附属第一医院泌尿外科,浙江宁波 315010
- Publication Type:Journal Article
- Keywords:
purinergic 2X7 receptor;
NOD-like receptor pyrin domain-containing 3;
inflammasome;
benign prostatic hy-perplasia
- From:
National Journal of Andrology
2025;31(11):986-992
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study is to detect the expression of purinergic 2X7 receptor(P2X7R)and the NLRP3 inflammasome in benign prostatic hyperplasia(BPH)tissues and to analyze the clinical correlations.Methods Twelve patients undergoing surgery for BPH were enrolled.Based on the presence or absence of inflammatory cell infiltration in HE-stained tissue sections,the patients were divided into inflammation group and non-inflammation group.Preoperative routine ex-aminations excluded surgical contraindications,and International Prostate Symptom Score(IPSS)questionnaires,urinary flow rate measurements,expressed prostatic secretions(EPS)analysis,and prostate-specific antigen(PSA)tests were conducted.Prostate tissues obtained during surgery were subjected to HE staining,immunofluorescence/Western blot to detect the expression of NLRP3,P2X7R,Caspase-1,Cleaved-Caspase-1,IL-1 β,and TNF-α,and immunofluorescence to assess lymphocyte infiltra-tion.SPSS 26.0 software was used to analyze correlations between the expression levels of NLRP3 and P2X7R in prostate tissues and indicators including Caspase-1,Cleaved-Caspase-1,IL-1 β,TNF-α,lymphocyte count,IPSS score,urinary flow rate,EPS,and PSA.Results In BPH tissues,the expression levels of NLRP3 and P2X7R were positively correlated(P＜0.05).The ex-pression levels of NLRP3 and P2X7R were positively correlated with Caspase-1,Cleaved-Caspase-1,IL-1 β,TNF-α,and lym-phocyte count(P＜0.05).NLRP3 and P2X7R expression levels were positively correlated with white blood cell count in EPS,but showed no correlation with IPSS score,lecithin body count in EPS,maximum urinary flow rate and PSA(P＜0.05).Conclusion P2X7R and NLRP3 in prostate tissues exacerbate local inflammatory responses,which may be an important mecha-nism in BPH development.However,they are not correlated with IPSS score,lecithin body count in EPS,maximum urinary flow rate and PSA.
