The reduction of reproductive function of male zebrafish caused by high-fat diet:A molecular basis study
10.13263/j.cnki.nja.2025.11.001
- VernacularTitle:高脂饮食致雄性斑马鱼生殖功能降低的分子基础研究
- Author:
Zhenghong DU
1
;
Fei MAO
;
Xue ZHAO
;
Shuo WANG
;
Yongjun WAN
;
Chengyu ZHANG
;
Peng DUAN
;
Dahu ZHANG
Author Information
1. 湖北医药学院附属襄阳市第一人民医院泌尿外科,湖北襄阳 441000;湖北医药学院附属襄阳市第一人民医院襄阳市斑马鱼建模与人类疾病药物筛选重点实验室,湖北襄阳 441000
- Publication Type:Journal Article
- Keywords:
high-fat diet;
zebrafish;
testis;
sperm motility;
transcriptomics
- From:
National Journal of Andrology
2025;31(11):963-971
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of high-fat diet(HFD)-induced obesity on male ze-brafish reproductive function.Methods Adult male zebrafish were divided into normal diet(ND)group and HFD group.Growth and metabolic conditions were evaluated by measuring body weight,body length,BMI,organ index,and glucose/lipid lev-els.Reproductive capacity was assessed via sperm concentration,motility,fertilization rate,and testosterone levels.Testicular tissues from both of groups were subjected to transeriptomic sequencing(RNA-seq).And qRT-PCR was used to validate the expression of genes.Results Compared to male zebrafish in ND group,the ones in HFD group exhibited hepatic steatosis and glucose/lipid meta-bolic disorders(P＜0.05).Testicular structural disorganization,along with reduced testosterone levels,decreased gonadosomatic in-dex,and impaired sperm concentration and motility occurred in HFD group(P＜0.05).GO analysis revealed that differentially ex-pressed genes were enriched in spermatogenesis and ciliary system,while KEGG analysis highlighted metabolic related pathways(pu-rine metabolism,thyroid hormone synthesis,mTOR signaling)and cell adhesion molecules.Twenty key differentially expressed genes were validated by qRT-PCR,which confirmed the reliability of RNA-Seq results.Conclusion Impairment of reproductive function induced by HFD in zebrafish may be associated with three regulatory mechanisms including ciliary system,metabolic dysregulation,and aberrant cell adhesion molecule signaling.This study provides mechanistic insights and identifies potential therapeutic targets for clinical management of diet-associated infertility.
