Effect of serum 25-hydroxyvitamin D3 level on ovarian reserve function and artificial insemination outcomes in infertile patients
10.3760/cma.j.cn101721-20240923-00302
- VernacularTitle:不孕症患者血清25（OH）D 3水平对卵巢储备功能及人工授精助孕结局的影响
- Author:
Xiaoning CUI
1
;
Zhengmei ZHANG
1
;
Huiling WANG
1
Author Information
1. 陕西省延安市人民医院妇产科，延安 716000
- Publication Type:Journal Article
- Keywords:
Infertility;
25-hydroxyvitamin D3;
Artificial insemination;
Ovarian function
- From:
Clinical Medicine of China
2025;41(2):88-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of serum 25-hydroxyvitamin D3 level on ovarian reservation function and artificial insemination outcomes in infertile patients.Methods:A retrospective analysis was conducted based on clinical data of 108 infertile patients who underwent artificial insemination at Yan'an People's Hospital from January 2021 to January 2024. The patients' baseline data and test results of laboratory indicators were collected. Patients enrolled were divided into vitamin D deficiency group (73 cases) and non-vitamin D deficiency group (35 cases) according to serum 25-hydroxyvitamin D3 [25(OH)D3] level, and divided into pregnant group (60 cases) and non-pregnant group (48 cases) according to artificial insemination outcome. Baseline data and laboratory indicators of different groups were compared in order to analyze factors that influence failure of artificial insemination in infertile patients. Measurement data with normal distribution was represented by ±s. Comparison between groups was performed by two-sample t-test. Enumeration data was represented by n(%). Comparison between groups was performed by χ2 test. Logistic regression analysis was used to identify risk factors of artificial insemination failure in infertile patients. Results:Serum 25(OH)D3 and anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) in the vitamin D deficiency group were lower than those in the non-vitamin D deficiency group [(14.9±2.6) μg/L vs. (22.6±2.1) μg/L, (3.0±1.0) μg/L vs. (3.4±1.0) μg/L, (9.9±2.3) vs. (11.1±2.6)], and the differences were statistically significant ( t=15.28, P<0.001; t=2.28, P=0.025; t=2.31, P=0.023). In the vitamin D deficiency group, serum 25(OH)D3 and AMH levels were positively correlated with AFC (r=0.40 and 0.37, both P<0.001). The proportion of retrieved oocytes ≥ 8, AFC, serum 25(OH)D3 and AMH levels in the pregnant group were higher than those in the non-pregnant group [56.7% (34/60) vs. 31.2% (15/48), (11.3±2.4) vs. (9.0±2.2), (23.2±2.2) μg/L vs. (10.1±2.0) μg/L, (3.5±1.1) μg/L vs. (2.6±0.7) μg/L], and the differences were statistically significant ( χ2=6.95, P=0.008, t=5.22, P<0.001; t=32.01, P<0.001; t=4.80, P<0.001). Multivariate logistic regression analysis results showed that low serum 25(OH)D3, AMH, and AFC were risk factors of artificial insemination failure in infertile patients (odds ratios=0.49, 0.56, 0.31, 95%CI: 0.25-0.96, 0.33-0.95, 0.11-0.87, P=0.037, 0.032, 0.026). Conclusions:Serum 25-hydroxyvitamin D3 level is closely related to ovarian reservation function in infertile patients. Moreover, it is a risk factor of artificial insemination failure.
