Yishen Shengyang formula ameliorates allergic rhinitis via Bach2-driven Treg cell regulation
10.3969/j.issn.1673-9701.2025.15.012
- VernacularTitle:益肾升阳方经Bach2 调控Treg细胞治疗变应性鼻炎
- Author:
Zhichao MA
1
;
Chaohui ZHANG
1
;
Yongjie YING
1
;
Yilong WANG
1
;
Qiaozhi JIN
1
;
Wubing CHEN
1
;
Baohong TAO
1
Author Information
1. 台州市立医院耳鼻咽喉科,浙江 台州 318000
- Publication Type:Journal Article
- Keywords:
Yishen Shengyang formula;
Allergic rhinitis;
Bach2;
Regulatory T cell;
Immune regulation
- From:
China Modern Doctor
2025;63(15):56-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects and mechanisms of Yishen Shengyang formula in allergic rhinitis(AR)by regulating the transcription factor BTB and CNC homology 2(Bach2)to promote regulatory T cell(Treg cell)differentiation.Methods AR mouse models were established via ovalbumin(OVA)sensitization and randomly divided into control group,AR model group(AR group),Yishen Shengyang formula group and dexamethasone group.Nasal symptoms were evaluated using behavioral scores;Histopathological changes in nasal mucosa were observed via hematoxylin-eosin and periodic acid Schiff stain;Serum levels of OVA-specific immunoglobulin E(sIgE),interleukin-4(IL-4),and transforming growth factor-β(TGF-β)were measured by enzyme linked immunosorbent assay;Splenic Treg proportions were analyzed via flow cytometry;Bach2 protein expression was assessed by Western blot.Results Compared with control group,AR group mice showed significantly increased behavioral scores(P<0.05),goblet cell hyperplasia,inflammatory infiltration,elevated OVA-sIgE and IL-4 levels(P<0.05),reduced TGF-β(P<0.05),and decreased splenic Treg proportions and Bach2 expression(P<0.05).Yishen Shengyang formula treatment alleviated nasal symptoms(P<0.05),mitigated mucosal damage,decreased OVA-sIgE and IL-4,increased TGF-β(P<0.05),and upregulated Treg proportions and Bach2 expression(P<0.05),with efficacy comparable to dexamethasone.Conclusion Yishen Shengyang formula demonstrates potential therapeutic effects on allergic rhinitis by upregulating Bach2 expression and promoting Treg cell differentiation.This study provides a novel strategy and experimental basis for the traditional Chinese medicine treatment of AR.
