Simultaneous content determination of sixteen constituents in Jiawei Huoxiang Zhengqi Soft Capsules by UPLC-MS/MS
10.3969/j.issn.1001-1528.2025.05.003
- VernacularTitle:UPLC-MS/MS法同时测定加味藿香正气软胶囊中16种成分的含量
- Author:
Qian WANG
1
;
Xia GAO
;
Jian FENG
;
Bin JIN
;
Xia-lin CHEN
;
Liang CAO
;
Ji-feng LI
;
Yong-wen ZHANG
;
Zhen-zhong WANG
Author Information
1. 中药制药过程控制与智能制造技术全国重点实验室(江苏康缘药业股份有限公司/南京中医药大学),江苏南京 210000;南京中医药大学,江苏南京 210023
- Publication Type:Journal Article
- Keywords:
Jiawei Huoxiang Zhengqi Soft Capsules;
chemical constituents;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(5):1431-1436
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of liquiritin,liquiritin apioside,verbascoside,narirutin,isoacteoside,apigetrin,hesperidin,isoliquiritin,ononin,liquiritigenin,glycyrrhizic acid,isoliquiritigenin,honokiol,obovatol,pogostone and magnolol in Jiawei Huoxiang Zhengqi Soft Capsules.METHODS The analysis was performed on a 40 ℃ thermostatic ZORBAX Eclipse Plus C18 column(2.1 mm×100 mm,1.8 μm),with the mobile phase comprising of 0.1％formic acid-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Sixteen constituents showed good linear relationships within their own ranges(r＞0.990 0),whose average recoveries were 83.74％-105.12％with the RSDs of 1.10％-4.8％.CONCLUSION This accurate,sensitive,stable and reproducible method can provide a reference for the overall quality control of Jiawei Huoxiang Zhengqi Soft Capsules.
