Chemical constituents from Radix Puerariae Lobatae from Anhui province and their in vitro lipid-lowering activities
10.3969/j.issn.1001-1528.2025.05.018
- VernacularTitle:皖产葛根化学成分及其体外降脂活性研究
- Author:
Jie YU
1
;
Zhu YANG
;
Qiu-yue LÜ
;
Guo-dong WANG
;
Jun HAN
;
Jiang-ping WU
Author Information
1. 皖南医学院药学院,安徽芜湖 241002;皖南医学院,新安医学与中医药现代化研究所,安徽芜湖 241002;安徽省皖南地区植物药活性物质筛选与再评价工程实验室,安徽芜湖 241002
- Publication Type:Journal Article
- Keywords:
Radix Puerariae Lobatae;
ethyl acetate fraction;
chemical constituents;
isolation and identification;
in vitro lipid-lowering activity
- From:
Chinese Traditional Patent Medicine
2025;47(5):1531-1539
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Radix Puerariae Lobatae from Anhui province and their in vitro lipid-lowering activity.METHODS D101 macroporous resin,silica gel,MCI,and semi-preparative HPLC were used for isolation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The lipid accumulation model was established by palmitic acid-induced hepatocyte AML12,and the in vitro lipid-lowering activity was evaluated.RESULTS Twenty-one compounds were isolated and identified as carboxymethyl isoferulate(1),methyl(E)feruloylglycolate(2),ariscucurbin-A(3),trans-p-coumaroygly colic acid(4),indole-3-carboxaldehyde(5),β-sitosterol(6),puerol A(7),pueroside C(8),cumoestrol(9),dihydrofurocoumarin(10),decuroside V(11),psoralen dimer(12),bergapten dimer(13),isoliquiritigenin(14),neoliquiritin(15),calycosin(16),daidzein(17),3,4,7-trihydroxyisoflavone(18),ononin(19),genistein(20),genistin(21).Compounds 1-4、7-10、13 and 17-19 significantly inhibited lipid accumulation in AML12 cells,and compound 9 inhibited PPAR-γ expression.CONCLUSION Compounds 1-6,10-13 are isolated from Pueraria genus for the first time.Compounds 1-4,7-10,13 and 17-19 have good lipid-lowering activity.
