Effect of moxibustion at Shenque(CV8)and Guanyuan(CV4)on TRPV1 channel in spinal dorsal horn and hypothalamus of dysmenorrhea rats
10.1007/s11726-025-1491-3
- VernacularTitle:艾灸神阙、关元对痛经大鼠脊髓背角和下丘脑TRPV1通道的影响
- Author:
Yanqiu SUN
;
Yulei LIANG
;
Di WANG
;
Zhiguo ZHAO
;
Liyun YANG
;
Xuanping ZHANG
;
Yan ZHANG
;
Xuan ZHANG
;
Yanxue XING
;
Min ZHOU
;
Xiaoyan WANG
;
Xinhua LI
;
Yanping YANG
- Publication Type:Journal Article
- Keywords:
Moxa Stick Moxibustion;
Point,Shenque(CV8);
Point,Guanyuan(CV4);
Dysmenorrhea;
Analgesia;
Central Nervous System Sensitization;
Transient Receptor Potential Vanilloid 1;
Rats
- From:
Journal of Acupuncture and Tuina Science
2025;23(2):117-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of mild moxibustion on transient receptor potential vanilloid type 1(TRPV1)channel expression in primary dysmenorrhea(PD)rats and explore its mechanism in alleviating central pain sensitization.Methods:Thirty-two female non-pregnant Wistar rats were randomized into a blank group,a model group,a mild moxibustion group,and a capsazepine group,with 8 rats in each group.Except for the blank group,the other three groups used estradiol benzoate,ice-water bath,and oxytocin to establish the rat PD model of cold-dampness stagnation pattern.The interventions began on day 1 of modeling,once a day,and lasted 10 d.The mild moxibustion group received mild moxibustion at Shenque(CV8)and Guanyuan(CV4),20 min/time;in the capsazepine group,capsazepine was injected at a dose of 2 mg/(kg·bw).The abdominal pain threshold was measured 10-30 min after oxytocin injection on day 11;enzyme-linked immunosorbent assay was used to detect serum prostaglandin F2α(PGF2α)level;the expression of TRPV1,cluster of differentiation 11B(CD11B),and proto-oncogene c-Fos in the spinal dorsal horn and hypothalamus was detected by immunofluorescence and Western blotting.Results:Compared to the blank group,the model group showed a decreased pain threshold(P<0.05)and an increased serum PGF2α level with elevated TRPV1,CD11B,and c-Fos protein expression in the spinal dorsal horn and hypothalamus(P<0.05).Compared to the model group,both the mild moxibustion group and capsazepine group showed significantly increased pain thresholds(P<0.05),along with decreased serum PGF2α levels and reduced protein expression levels of TRPV1,CD11B,and c-Fos in the spinal dorsal horn and hypothalamus(P<0.05).Rat pain threshold in the capsazepine group was higher than that in the mild moxibustion group(P<0.05).Serum PGF2α level,the expression levels of CD11B and c-Fos proteins in the spinal dorsal horn,as well as TRPV1,CD11B,and c-Fos proteins in the hypothalamus of the capsazepine group were lower than those in the mild moxibustion group(P<0.05).Conclusion:Mild moxibustion at Shenque(CV8)and Guanyuan(CV4)may alleviate the central pain sensitization in PD rats by down-regulating TRPV1 channel expression in the spinal dorsal horn and hypothalamus,thus playing an analgesic effect.
