Study on Quality Markers of Shugan Liqi Mixture Based on Characteristic Chromatogram,Serum Pharmacochemistry and Network Pharmacology
10.19879/j.cnki.1005-5304.202503429
- VernacularTitle:基于特征图谱、血清药物化学及网络药理学研究疏肝理气合剂质量标志物
- Author:
Jiayu MEI
1
;
Tianting NI
;
Yueying ZHANG
;
Desen YANG
;
Guoping GAN
Author Information
1. 湖北中医药大学药学院,湖北 武汉 430065
- Publication Type:Journal Article
- Keywords:
Shugan Liqi Mixture;
quality markers;
characteristic chromatogram;
serum pharmacochemistry;
network pharmacology
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(8):131-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the key quality markers of Shugan Liqi Mixture in the treatment of liver depression and qi stagnation and menstrual irregularities;To provide a scientific basis for the selection of index components in the process of its quality standardization study.Methods The characteristic chromatogram of Shugan Liqi Mixture was established by HPLC;the serum components of Shugan Liqi Mixture were analyzed by LC-MS;the results of the characteristic chromatogram and serum chemistries were integrated,and the network pharmacology was used to construct a component-target network of Shugan Liqi Mixture,so that important components related to liver depression and qi stagnation and menstrual dysfunction could be obtained.The key quality markers of Shugan Liqi Mixture were screened out by combining with relevant literature reports.Results A total of 12 common peaks were obtained from the characteristic chromatogram of 15 batches of Shugan Liqi Mixture,and 7 components were identified,including paeoniflorin,hyperoside,naringin,hesperidin,neohesperidin,baicalin and saikosaponin b2.Totally 26 prototype blood components,including isorhamnetin,nobiletin and tetrahydropalmatine,were identified by serum pharmacochemistry study.According to the"five principles"of quality markers,and combining the characteristic chromatogram and serum medicinal chemistry results,the five components of baicalin,naringin,tetrahydropalmatine,paeoniflorin and saikosaponin a were finally identified as the key quality markers of Shugan Liqi Mixture combined with the data from network pharmacology and the literature.Conclusion This study preliminarily identifies five key quality markers of Shugan Liqi Mixture,providing index components in the process of its quality standard research.
