Screening of material basis for Rhei Radix et Rhizoma on improving human umbilical vein endothelial cell injury before and after carbonization based on spectrum-effect relationship
10.3969/j.issn.1001-1528.2025.07.005
- VernacularTitle:基于谱效关系筛选大黄炭制前后改善人脐静脉内皮细胞损伤的物质基础
- Author:
Ting ZHAI
1
;
Li-tao LU
;
Jiang-wei HE
;
Ya-fei GUO
;
Mei GUO
;
Xin-yu ZHU
Author Information
1. 甘肃中医药大学,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
Rhei Radix et Rhizoma;
Rhei Radix et Rhizoma charcoal;
human umbilical vein endothelial cells(HUVECs);
material basis;
HPLC fingerprints;
hemostatic efficacy;
spectrum-effect relationship
- From:
Chinese Traditional Patent Medicine
2025;47(7):2163-2171
- CountryChina
- Language:Chinese
-
Abstract:
AIM To screen the material basis for Rhei Radix et Rhizoma on improving human umbilical vein endothelial cell(HUVECs)injury before and after carbonization.METHODS The HPLC fingerprints were established,after which difference analysis was performed by orthogonal partial least squares discriminant analysis.The LPS-induced HUVECs injury model was established,then NO,MDA levels and SOD activity were detected.Gray correlation analysis and partial least squares regression were adopted in the investigation of spectrum-effect relationship,and active constituents were screened.RESULTS There were 22 and 20 common peaks in the fingerprints for 17 batches of raw products and carbonized products,respectively,along with the similarities of more than 0.8.Twelve main difference components were observable,among which gallic acid,5-hydroxymethylfurfural,catechin,aloe rhodopsin-8-O-β-D-glucoside,rhubarbic acid-8-O-β-D-glucoside and sennosides A were identified.The carbonized products demonstrated stronger effect on improving HUVECs injury than the raw product.The correlations of common peaks were more than 0.5 in the fingerprints for raw products and carbonized products,and peaks 3,5,11,12,15,19,23 exhibited significant effects on their efficacy(VIP values＞1).CONCLUSION This accurate,reliable and reproducible method can provide a basis for clarifying the material basis for hemostatic efficacy of Rhei Radix et Rhizoma and its carbonized product.
