Simultaneous residue determination and exposure risk assessment of eleven plant growth regulators in Renshen Guben preparations by HPLC-MS/MS
10.3969/j.issn.1001-1528.2025.07.019
- VernacularTitle:HPLC-MS/MS法同时测定人参固本制剂中11种植物生长调节剂残留量及暴露风险评估
- Author:
Wei-kun ZHU
1
;
Jing WANG
;
Guo-jing QU
;
Yan-juan LIU
;
Xi-bo DOU
;
Dan-tong ZHAO
Author Information
1. 菏泽市食品药品检验检测研究院,山东菏泽 274000
- Publication Type:Journal Article
- Keywords:
Renshen Guben preparations;
plant growth regulators;
residues;
HPLC-MS/MS;
exposure risk assessment
- From:
Chinese Traditional Patent Medicine
2025;47(7):2255-2262
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-MS/MS method for the simultaneous residue determination of 11 plant growth regulators(PGRs)in Renshen Guben preparations,and to conduct a risk assessment.METHODS The analysis was performed on a 40 ℃ thermostatic ACQUITY UPLC ? Waters HSS T3 column(2.1 mm×100 mm,1.8 μm),with the mobile phase of acetonitrile-0.1％formic acid(containing 5 mmol/L ammonium formate)flowing at 0.30 mL/min in a gradient elution manner,and electro spray ionization was employed in both positive and negative ion scanning,with multiple reaction monitoring mode.The chronic and acute exposure risk values of the detected PGRs were calculated and assessed based on residue levels,health guidance values,and exposure estimates.RESULTS Eleven PGRs exhibited good linear relationships within their own ranges(R2 ≥ 0.990),whose average recoveries were 70.0％-120.0％,with RSDs all below 12.0％.In both oral liquid and pill forms,mepiquat chloride showed the highest average residue levels,while sodium 5-nitroguaiacolate exhibited the highest acute risk value(0.765 7,0.908 1)and chronic risk value(0.023 1,0.027 0).CONCLUSION Although PGRs residues are detected in Renshen Guben preparations,all levels remained within safe limits.
