Chemical constituents from the sticks and leaves of Croton cascarilloides and their biological activities
10.3969/j.issn.1001-1528.2025.07.018
- VernacularTitle:银叶巴豆枝叶化学成分及其生物活性研究
- Author:
Yu-jie LÜ
1
;
Hui-qin CHEN
;
Hao WANG
;
Jing-zhe YUAN
;
Wen-li MEI
;
Shou-bai LIU
;
Hao-fu DAI
Author Information
1. 海南大学热带农林学院,热带特色林木花卉遗传与种质创新教育部重点实验室,海南海口 570228;中国热带农业科学院热带生物技术研究所,海南省黎药资源天然产物研究与利用重点实验室,海南海口 571101
- Publication Type:Journal Article
- Keywords:
Croton cascarilloides Raeusch.;
sticks and leaves;
chemical constituents;
isolation and identification;
anti-inflammatory activity;
gastric mucosal protection activity
- From:
Chinese Traditional Patent Medicine
2025;47(7):2249-2254
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from the sticks and leaves of Croton cascarilloides Raeusch.and their biological activities.METHODS The 95％ethanol extract from the sticks and leaves of C.cascarilloides was isolated and purified by MCI,silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.LPS-induced NO RAW264.7 cell model induced by LPS was used to evaluate its anti-inflammatory activity in vitro.GES-1 injury model induced by taurocholic acid was used to screen the gastric mucosal protection activity.RESULTS Fourteen compounds were isolated and identified as bullatantriol(1),(-)-boscialin(2),(+)-dehydrovomifoliol(3),3-(hydroxylacetyl)-indole(4),pinoresinol(5),3,7-dimethyl-octa-1,7-diene-3,6-ol(6),(+)-syringaresinol(7),curcasinlignan B(8),cleomiscosin C(9),cleomiscosinD(10),2,6-dimethyl-octa-1,7-dien-3,6-diol(11),vanillin(12),vanillic acid(13),methyl vanillate(14).Compound 4 had certain anti-inflammatory activity,with IC50 values of 73.62 μmol/L.The protective rates of 25 μmol/L compounds 1-4,6,9-12 and 14 on gastric mucosal epithelial cells were 30.07％,34.18％,23.91％,30.92％,17.51％,19.69％,31.76％,22.46％,30.56％and 14.49％,respectively.CONCLUSION Compounds 1-14 are isolated from this plant for the first time.Compound 4 shows anti-inflammatory activity,1-4,6,9-12 and 14 show different degrees of gastric mucosal epithelial cell protective activity.
