Simultaneous content determination of eleven constituents in Bunao Soft Capsules by HPLC
10.3969/j.issn.1001-1528.2025.07.002
- VernacularTitle:HPLC法同时测定补脑软胶囊中11种成分的含量
- Author:
Ting CHEN
1
;
Xue-mei WANG
;
Shuai-yin LI
;
Xi-xiang LI
;
Ya-li ZHOU
;
Xiao-feng LI
;
Shou-yuan YANG
Author Information
1. 甘肃中医药大学,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
Bunao Soft Capsules;
chemical constituents;
content determination;
HPLC
- From:
Chinese Traditional Patent Medicine
2025;47(7):2144-2148
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of 5-hydroxymethylfurfural,chlorogenic acid,caffeic acid,strychnine,paeoniflorin,ferulic acid,paeoniflorin Ⅰ,epimedium glycoside,psoralen,isopsoralen and glycyrrhetinic acid in Bunao Soft Capsules.METHODS The analysis was performed on a 35 ℃ thermostatic Waters Symmetry C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 230,280 nm.RESULTS Eleven constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 98.47％-103.30％with RSDs of 1.13％-2.80％.CONCLUSION This simple and reliable method can be used for the quality control of Bunao Soft Capsules.
