C1q-like protein 4 promotes invasion and metastasis of breast cancer BT549 cells by regulating the NF-κB signaling pathway
10.3781/j.issn.1000-7431.2025.2401-0059
- VernacularTitle:C1q样蛋白4通过调控NF-κB信号转导通路促进乳腺癌BT549细胞的侵袭和转移
- Author:
Xiao LI
1
;
Bo ZHAO
1
;
Qing ZHANG
1
;
Yi DONG
1
;
Fan XU
1
Author Information
1. 承德医学院附属医院肿瘤科,河北 承德 067000
- Publication Type:Journal Article
- Keywords:
Breast cancer;
C1ql4;
Migration;
Invasion;
Nuclear factor-κB;
Epithelial-mesenchymal transition
- From:
Tumor
2025;45(1):12-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of C1q like protein 4(C1ql4)on migration and invasion of breast cancer and its mechanism.Methods:Real-time fluorescence quantitative PCR was used to detect the expression level of C1ql4 mRNA in tumor tissues and paracancerous tissues from 50 cases of breast cancer,as well as the breast cells including T549 and MCF-7 breast cancer cells,and MCF-10A normal breast cells.The siRNA targeting C1ql4 gene(siC1ql4)was synthesized and transfected into BT549 breast cancer cells by liposome transfection,and the silencing efficiency was detected by Western blotting.MTT assay was used to detect the proliferative ability of the cells in each group,the invasive ability of the cells was detected by Transwell assay,and the migratory ability of the cells was detected by scratch healing assay.Western blotting was used to detect the proliferative ability of the cells in each group.The expression levels of Snail,Slug,nuclear factor-κB(NF-κB)and phospho-NF-κB(p-NF-κB)were detected.The immunofluorescence was used to localize the distribution of NF-κB.Real-time fluorescence PCR was used to detect the mRNA expression levels of the NF-κB target genes TNF-α and IL-1β.Results:The expression level of C1ql4 mRNA in breast cancer tissues was significantly higher than that in paracancerous tissues,and the expression level of C1ql4 mRNA in BT549 and MCF-7 breast cancer cells was significantly higher than that in MCF-10A normal breast cells(P<0.05).After silencing C1ql4 expression,the proliferation,invasion and migration abilities of BT549 cells were significantly decreased(all P<0.001);the mRNA and prorein expression levels of Snail and Slug were significantly down-regulated(both P<0.001),and the mRNA expression levels of TNFα and IL-1β were significantly reduced(both P<0.001).The amount of NF-κB entering the nucleus was reduced,and the ratio of p-NF-κB to NF-κB was reduced(both P<0.05).Conclusion:The C1ql4 gene may regulate migration and invasion of breast cancer cells and promote breast cancer progression through the NF-κB pathway.
