Mechanism of low-intensity pulsed ultrasound for promoting osteogenic differentiation of senescent bone marrow mesenchymal stem cell in mice
10.13929/j.issn.1003-3289.2025.04.012
- VernacularTitle:低强度脉冲超声促进小鼠衰老骨髓间充质干细胞成骨分化作用机制
- Author:
Shiwen HE
1
;
Ke YANG
;
Huan YAO
;
Shujin YAN
;
Li TANG
;
Zhigang WANG
Author Information
1. 重庆医科大学附属第二医院超声科 超声影像学研究所 超声分子影像诊疗重庆市重点实验室,重庆 400010
- Publication Type:Journal Article
- Keywords:
osteoporosis;
bone marrow cell;
low-intensity pulsed ultrasound;
osteogenic differentiation;
myocardin-related transcription factor
- From:
Chinese Journal of Medical Imaging Technology
2025;41(4):563-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the capacity of low-intensity pulsed ultrasound(LIPUS)for promoting osteogenic differentiation of senescent bone marrow mesenchymal stem cell(BMMSC)in mice,also the potential mechanism of involving myocardin-related transcription factor-A(MRTF-A)during this process.Methods Five aged mice received LIPUS irradiation on the left femur for eight weeks.Micro CT and HE staining were employed to evaluate bone status of bilateral femur.Bone marrow cells were isolated from mouse long bones,BMMSC were cultured,and the cells were divided into H2O2+LIPUS group,H2O2 group and control group.Mouse BMMSC were subjected to H2O2 for 3 days,followed by reactive oxygen species fluorescence staining and senescence-associated β-galactosidase staining to assess cellular senescence.Immunofluorescence and phalloidin staining were performed to examine MRTF-A distribution and cytoskeletal status.Based on H2O2-induced senescence model,MRTF-A nuclear translocation inhibitor CCG-100602 was applied,and the cells were further divided into H2O2+CCG-100602+LIPUS group,H2O2+CCG-100602 group,H2O2+LIPUS group and H2O2 group.Immunofluorescence staining was used to assess MRTF-A distribution.Alkaline phosphatase(ALP)and alizarin red staining were conducted to evaluate osteogenic differentiation capacity.Results LIPUS resulted in increased trabecular bone in the left femur of aged mice compared to the right femur,with increased bone volume to total volume and trabecular number and decreased trabecular spacing(both P＜0.05).Compared with H2O2 group,H2O2+LIPUS group showed increased nuclear expression of MRTF-A and increased intracellular rigid actin filament density.The nuclear expression of MRTF-A in H2O2+CCG-100602+LIPUS group was significantly lower than that in H2O2+LIPUS group.Compared with the H2O2+CCG-100602+LIPUS group and H2O2 group,the ALP and alizarin red staining positive areas in H2O2+LIPUS group were significantly larger.Conclusion LIPUS could improve osteoporosis in aged mice.Nuclear translocation of MRTF-A was a key regulator to LIPUS for promoting osteogenic differentiation of senescent BMMSC.
