Exploration on the Mechanism of Chaipo Decoction in Intervening Acute Lung Injury Based on Network Pharmacology and Experimental Verification
10.19879/j.cnki.1005-5304.202501036
- VernacularTitle:基于网络药理学与实验验证探讨柴朴汤干预急性肺损伤机制
- Author:
Da ZHAO
1
;
Tianyu ZHANG
;
Zhenqi WU
;
Guanghua LIU
Author Information
1. 辽宁中医药大学,辽宁 沈阳 110847
- Publication Type:Journal Article
- Keywords:
Chaipo Decoction;
acute lung injury;
network pharmacology;
molecular docking;
PI3K/Akt signaling pathway;
NF-κB
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(6):38-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Chaipo Decoction in intervening lipopolysaccharide(LPS)-induced acute lung injury(ALI)based on network pharmacology and experimental verification.Methods TCMSP database was used to screen the active components and targets of Chaipo Decoction.ALI disease targets were retrieved through GeneCards and OMIM databases,the intersection genes of drug and disease were obtained.They were imported into STRING platform and Cytoscape 3.9.1 software to construct protein interaction network and drug-active components-target network,core components and targets were screened,and GO and KEGG pathway enrichment analysis was performed on them.AutoDock 1.5.6 and PyMOL software were used to verify the molecular docking between the main active components and core targets.The ALI model of mice was induced by LPS,and was intervened by Chaipo Decoction.The pathological changes of lung tissue in mice were observed by HE staining.The contents of IL-6,IL-10 and TNF-α in serum of mice were detected by ELISA.Western blot was used to verify the expressions of target proteins in lung tissue.Results Totally 214 active components of Chaipo Decoction,such as norwogonin,baicalein,quercetin and ursolic acid were screened,with 1 101 targets and 271 potential targets for intervention in ALI,mainly SRC,STAT3,AKT1,EGFR,GRB2,PIK3CA,etc.It mainly affected PI3K/Akt signaling pathway,MAPK signaling pathway,apoptosis,Ras signaling pathway,etc.Molecular docking showed that the main active components of Chaipo Decoction had strong binding ability with core targets SRC,STAT3,AKT1,EGFR and GRB2.The results of animal experiments showed that Chaipo Decoction could alleviate the lung lesions in ALI mice,decrease the contents of IL-6 and TNF-α in serum,increase the content of IL-10,and significantly decreased the expressions of EGFR,PI3K,AKT and NF-κB p65 protein in lung tissue.Conclusion Chaipo Decoction can exert anti-ALI effect through multiple pathways and multiple targets,and its mechanism may be related to the inhibition of EGFR/PI3K/Akt signaling pathway.
