Exploration on the Mechanism of Bufei Jiedu Granules in Treating MRSA Chronic Infection Based on Network Pharmacology and Experimental Verification
10.19879/j.cnki.1005-5304.202411489
- VernacularTitle:基于网络药理学和实验验证探讨补肺解毒颗粒治疗MRSA慢性感染作用机制
- Author:
Ruoqing PENG
1
;
Peiyong ZHENG
1
;
Wei ZHOU
1
;
Yuwei JIANG
1
;
Ben SU
1
;
Xianwei WU
1
;
Lei QIU
1
;
Shaoyan ZHANG
1
;
Zhenhui LU
1
Author Information
1. 上海中医药大学附属龙华医院呼吸疾病研究所,上海 200030
- Publication Type:Journal Article
- Keywords:
Bufei Jiedu Granules;
MRSA infection;
network pharmacology;
immune cells
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(6):29-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Bufei Jiedu Granules in the treatment of methicillin-resistant Staphylococcus aureus(MRSA)chronic infection using network pharmacology;To verify it through animal experiments.Methods Active components and potential targets in Bufei Jiedu Granules were screened through the TCMSP database,and genes related to MRSA infection were retrieved through GeneCards,OMIM,DisGeNET,TTD,DrugBank and PharmGKB databases.The STRING database was employed to construct a protein-protein interaction network on common targets,and GO and KEGG pathway enrichment analysis were conducted to identify key signaling pathways for Bufei Jiedu Granules treatment of MRSA infection.The effects of Bufei Jiedu Granules on bacterial load and pathological changes in the lung,liver and kidney of MRSA chronic infection mice models were evaluated through WT and T/B immune cell deficient Rag2-/-mouse animal experiments.The mRNA expressions of inflammatory factors(IFN-γ,IL-10)and immune checkpoints(PD1,TIM3)were detected.Results Totally 54 active components related to Bufei Jiedu Granules were selected,and 50 potential targets related to MRSA infection were identified.118 signaling pathways significantly associated with MRSA infection were identified through GO and KEGG pathway enrichment analysis,in which the JAK-STAT signaling pathway,Th17 cell differentiation,and PD-L1 expression and PD-1 checkpoint pathway were closely related to cell activation and T cell differentiation.Animal experimental results indicated that Bufei Jiedu Granules could effectively reduce the bacterial load in organs and ameliorate the pathological damage in the chronic MRSA infection mouse model,increase the mRNA expression of IFN-γ in the lung tissue,and decrease the mRNA expressions of IL-10,PD1 and TIM3.Conclusion Bufei Jiedu Granules has the characteristics of multi-component and multi-target in the treatment of MRSA infection,and may be involved in adaptive immune activation to effectively treat chronic MRSA infection.
