Exploration on the Intervention Mechanism of Bushen Zhupai Decoction on Polycystic Ovary Syndrome Rats with Insulin Resistance Based on IL-6/PI3K/AKT Signaling Pathway
10.19879/j.cnki.1005-5304.202408031
- VernacularTitle:基于IL-6/PI3K/AKT信号通路探讨补肾助排汤对多囊卵巢综合征伴胰岛素抵抗大鼠的干预机制
- Author:
Wenjuan LIN
1
;
He LI
;
Shouyue RUI
;
Cancan HUANG
;
Wenwen WAN
;
Rongrong YUAN
;
Shuyuan ZHANG
;
Xiangyu LIN
;
Xiaohua ZHANG
Author Information
1. 甘肃中医药大学,甘肃 兰州 730000
- Publication Type:Journal Article
- Keywords:
polycystic ovary syndrome;
Bushen Zhupai Decoction;
insulin resistance;
IL-6/PI3K/AKT signaling pathway;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(6):121-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intervention mechanism of Bushen Zhupai Decoction on polycystic ovary syndrome rats with insulin resistance based on IL-6/PI3K/AKT signaling pathway Methods A rat model of polycystic ovary syndrome with insulin resistance was established by feeding high-fat diet combined with oral administration of letrozole,and the blank group was fed with ordinary diet.The modeled rats were randomly divided into model group,metformin group and Bushen Zhupai Decoction low-,medium-and high-dosage groups.The corresponding intervention were given for 28 days.Fasting blood glucose(FBG)content was detected,and insulin resistance index(HOMA-IR)was calculated.ELISA was used to detect serum fasting insulin(FINS),testosterone(T),estradiol(E2),luteinizing hormone(LH)and follicle stimulating hormone(FSH)contents.HE staining was used to observe the morphology of ovarian tissue.Immunohistochemistry was used to detected the expressions of interleukin(IL)-6,p-PI3K,p-AKT and GLUT4 in ovarian tissue.Western blot was used to detect the protein expressions of IL-6,PI3K,p-PI3K,AKT,p-AKT and GLUT4 in ovarian tissue,while RT-PCR was used to detect the mRNA expressions of IL-6,PI3K,AKT and GLUT4.Results Compared with the blank group,the body mass,FBG,FINS and serum T and LH contents of the model group rats significantly increased(P<0.01),while the serum E2 and FSH contents significantly decreased(P<0.01);ovarian tissue showed polycystic changes,with reduced layers and loose arrangement of granulosa cells,the expression of IL-6 protein and mRNA in ovarian tissue significantly increased(P<0.01),the expressions of p-PI3K,p-AKT and GLUT4 proteins significantly decreased(P<0.01),and the expressions of PI3K,AKT and GLUT4 mRNA significantly decreased(P<0.01).Compared with the model group,the body mass of rats in each administration group decreased,and the contents of FBG,FINS and serum T and LH decreased(P<0.01,P<0.05),while the contents of E2 and FSH increased;polycystic changes in ovarian tissue were reduced,mature follicles increased,and granulosa cells were tightly arranged,the expression of IL-6 protein and mRNA in ovarian tissue decreased,the expressions of p-PI3K,p-AKT,GLUT4 protein and PI3K,AKT,GLUT4 mRNA increased.Bushen Zhupai Decoction high-dosage group showed statistical significance(P<0.01).Conclusion Bushen Zhupai Decoction can improve reproductive and metabolic disorders in polycystic ovary syndrome with insulin resistance rats,and its mechanism may be related to inhibiting IL-6 expression,activating the PI3K/AKT signaling pathway,and alleviating insulin resistance.
