Molecular Identification of Cremastra appendiculata and Other Original Medicinal Materials of Cremastrae Pseudobulbus and Confusing Products Based on ITS Sequences in DNA Barcodes
10.19879/j.cnki.1005-5304.202502180
- VernacularTitle:基于DNA条形码ITS序列的杜鹃兰与山慈菇其他基原药材及混伪品分子鉴定
- Author:
Jinling ZHANG
1
;
Qiuxiao XIAO
;
Qian WANG
;
Sijia ZHOU
;
Yali LONG
;
Yong HUANG
;
Lin ZHENG
;
Zipeng GONG
;
Yang JIN
;
Yueting LI
Author Information
1. 贵州医科大学,中药功效成分发掘与利用全国重点实验室,民族药与中药开发应用教育部工程研究中心,贵州省民族药与中药开发应用工程技术研究中心,贵州 贵阳 550004;贵州医科大学药学院,贵州 贵阳 550004
- Publication Type:Journal Article
- Keywords:
Cremastra appendiculata;
Cremastrae Pseudobulbus;
ITS sequence;
DNA barcodes;
molecular identification
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(6):142-146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To distinguish Cremastra appendiculata(D.Don)Makino,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth using the ITS sequence in DNA barcodes;To explore the genetic diversity of Cremastra appendiculata germplasm resources.Methods Three different original Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and their easily confusing products Cremastrae Pseudobulbus of Oreorchis patens and Iphigenia indica Kunth were selected as the research objects,and the genomic DNA of the above samples were extracted by the modified CTAB method,and then the ITS sequences were amplified,sequenced and spliced by PCR technology.The Kimura 2-Parameter(K2P)model was used to calculate the genetic distance,and the phylogenetic tree was constructed with the help of neighbour joining method(NJ)for genetic relationship analysis.Results Except for the Iphigenia indica Kunth species that were not found during the BLAST search,the BLAST comparison results of the other samples were higher than 95%.At the same time,the results of phylogenetic tree showed that Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides were clustered into one branch,respectively,and the easily confusing products were also respectively clustered into one branch.Conclusion The ITS sequence in DNA barcodes can be used to accurately distinguish Cremastra appendiculata,Pleione yunnanensis Rolfe and Pleione bulbocodioides,and its easily confusing products Oreorchis patens and Iphigenia indica Kunth.
