The mechanism study of PD-L2 influencing metastasis of head and neck squamous cell carcinoma via regulating EMT
10.12354/j.issn.1000-8179.2025.20250409
- VernacularTitle:PD-L2调控EMT影响头颈部鳞状细胞癌转移的机制研究
- Author:
Yu XIAOXUE
1
;
Sun MENGYU
;
Liu CHAO
;
Zheng JIANWEI
;
Mei MEI
;
Zhou XUAN
;
Ren YU
Author Information
1. 天津医科大学基础医学院遗传学系(天津市 300070)
- Publication Type:Journal Article
- Keywords:
head and neck squamous cell carcinoma(HNSCC);
programmed death-ligand 2(PD-L2);
epithelial-mesenchymal transition(EMT);
metastasis
- From:
Chinese Journal of Clinical Oncology
2025;52(11):550-556
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to investigate the characteristic expression of programmed death-ligand 2(PD-L2)and epithelial-mesenchymal transition(EMT)-related biomarkers in head and neck squamous cell carcinoma(HNSCC)and their mechanism of action in HNSCC metastasis.Methods:Tumor tissue samples from 94 patients with HNSCC were collected from Tianjin Medical University Cancer Hos-pital from January 2018 to July 2023,and their correlation with clinicopathological parameters,including lymph node metastasis,was statist-ically assessed.Western blot was used to detect PD-L2 expression in HNSCC tumor tissues.PD-L2 overexpression and knockdown stably-transfected monoclonal cell lines were generated using lentiviral vectors.Transwell assays were performed to explore the effect of PD-L2 on the invasive migration of the HNSCC cell lines.Additionally,RNA sequencing was used to identify downstream target genes regulated by PD-L2.The expression levels of key EMT markers,including E-cadherin,N-cadherin,and Vimentin,were examined by Western blot to elucidate the molecular mechanism by which PD-L2 promotes tumor cell metastasis through EMT pathway activation.Additionally,RNA sequencing was employed to identify downstream target genes regulated by PD-L2.The expression levels of key EMT markers,including E-cadherin,N-cadherin,and Vimentin,were examined with Western blot analysis to elucidate the molecular mechanism by which PD-L2 promotes tumor cell metastasis through the EMT pathway activation.Results:High expression of PD-L2 is positively correlated with N staging(P<0.05),and elevated PD-L2 expression predicts a poor prognosis in HNSCC patients.Conclusions:PD-L2 regulates the EMT pathway to promote HNSCC metastasis.Targeting PD-L2 is expected to provide a new strategy for the treatment of metastatic HNSCC.
