Regulatory role and mechanism of lobetyolin in the proliferation and apoptosis of brain glioma cells
10.3969/j.issn.1009-0126.2025.07.025
- VernacularTitle:党参炔苷对脑胶质瘤细胞增殖与凋亡的调控作用及机制
- Author:
Ming LIU
1
;
Yin ZHANG
1
;
Yongda LIU
1
;
Xiufeng ZHANG
1
;
Jianxin QIAO
1
;
Xiaosong FENG
1
;
Xipeng LIU
1
Author Information
1. 075000 张家口,河北北方学院附属第一医院神经外科
- Publication Type:Journal Article
- Keywords:
lobetyolin;
proto-oncogene proteins c-akt;
glycogen synthase kinase 3 beta;
glioma
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2025;27(7):952-958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory role and mechanism of lobetyolin(LBT,a poly-acetylene glycoside isolated from the roots of Codonopsis pilosula)in the proliferation and apop-tosis of brain glioma cells based on the Akt/GSK-3β/Snail signaling pathway.Methods Human brain glioma cell line U-373MG was randomly divided into normal,SC79(Akt activator),LBT,and LBT+SC79 groups.After corresponding interventions,CCK-8 assay,colony formation assay,and flow cytometry were used to detect the proliferation and apoptosis of the cells.Western blot-ting was employed to measure the protein expression levels of the molecules related to prolifera-tion,apoptosis,and Akt/GSK-3β/Snail signaling pathway.After tumor xenograft nude mouse model of U-373MG cells was established,followed by grouping and interventions as above cell experiments,the tumor weight and volume were measured.Immunohistochemical assay and TUNEL assay were performed to detect the proliferation and apoptosis of tumor cells.Western blotting was applied to detect Akt/GSK-3β/Snail signaling pathway related proteins in the nude mouse groups.Results In the LBT+SC79 group,cell viability,number of formed colonies,pro-tein levels of cyclin D1,Bcl-2 and Snail,p-Akt/Akt and p-GSK-3β/GSK-3β,tumor weight and vol-ume,and positive ratios of Ki67,cyclin D1 and Bcl-2 in transplanted tumors were increased(P＜0.05),and cell apoptotic rate[(3.20±1.14)％vs(46.15±1.52)％,P＜0.05],Bax protein level(0.51±0.07 vs 0.89±0.06,P＜0.05),and positive ratios of TUNEL[(51.56±7.13)％vs(74.95±8.61)％,P＜0.05]and Bax[(32.71±5.43)％vs(41.86±4.90),P＜0.05]in transplanted tumors were declined when compared with the LBT group.Conclusion LBT can induce apoptosis and inhibit proliferation of brain glioma cells in vitro and in vivo by blocking activation of the Akt/GSK-3β/Snail signaling pathway.
