Effect of Baoyuan Jiedu Decoction on Liver Lipids and Liver Mitochondrial Function in Cancer Cachexia Mice
10.16466/j.issn1005-5509.2025.02.001
- VernacularTitle:保元解毒汤对癌性恶病质小鼠肝脏脂质及肝脏线粒体功能的影响
- Author:
Li LUO
1
;
Shengqi HUANG
;
Yang WANG
Author Information
1. 浙江中医药大学基础医学院 杭州 310053
- Publication Type:Journal Article
- Keywords:
cancer cachexia;
Baoyuan Jiedu Decoction;
liver;
multi-dimensional mass spectrometry-based shotgun lipidomics;
lipid metabolism;
mitochondria;
mitochondrial respiratory chain complex;
medroxyprogesterone acetate
- From:
Journal of Zhejiang Chinese Medical University
2025;49(2):123-131
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the effect of Baoyuan Jiedu Decoction(BJD)on liver lipids and liver mitochondrial function in cancer cachexia mice.[Methods]The mice model of cancer cachexia was established by subcutaneous inoculation of Lewis lung cancer cells,and the mice were randomly divided into normal group,model group,BJD group and medroxyprogesterone acetate(MPA)group,which were given continuously for 21 days,and the food intake,body weight and tumor volume of the mice were recorded.Multi-dimensional mass spectrometry-based shotgun lipidomics(MDMS-SL)was used to detect liver lipid content.Changes of liver mitochondria were observed by transmission electron microscope.Enzyme-linked immunosorbent assay(ELISA)was used to detect adenosine triphosphate(ATP)content in liver.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression levels of liver mitochondrial respiratory chain complexes NADH:ubiquinone oxidoreductase subunit B8(NDUFB8),succinate dehydrogenase complex iron sulfur subunit B(SDHB)and ubiquinol-cytochrome-c reductase complex core protein 2(UQCRC2).The expression level of liver mitochondrial respiratory chain complex NDUFB8 protein was detected by Western blot.[Results]Compared with model group,the food intake and body weight of BJD group increased,and the tumor growth was slow(P<0.05).The results of lipidomics showed that the liver lipids in model group changed significantly compared with those in normal group,and BJD could obviously regulate 33 lipid molecules after intervention(P<0.05).The transmission electron microscope showed that the morphology of mitochondria in the model group was distorted,some mitochondrial contents were degraded,the outer membrane of mitochondria was broken,and the crista of mitochondria was obviously shortened and broken.Compared with the model group,the morphology of mitochondria in Baoyuan Jiedu Decoction group was obviously relieved,and the damage of crista structure was alleviated.The results of ELISA showed that the ATP content in the liver of BJD group was significantly higher than that of model group(P<0.05).RT-qPCR results showed that BJD could significantly increase the mRNA expression levels of mitochondrial respiratory chain complexes NDUFB8,SDHB and UQCRC2 after intervention(P<0.05).Western blot results showed that BJD group could significantly increase the protein expression level of mitochondrial respiratory chain complex NDUFB8 after intervention(P<0.05).[Conclusion]BJD can obviously increase the body weight and food intake of cancer cachexia mice,regulate the abnormal changes of liver lipid metabolism,restore the activity of liver mitochondrial respiratory chain complex,and improve mitochondrial respiratory function and energy metabolism.
