Characterization of microbiota in diarrhea-predominant irritable bowel syndrome based on 16S rDNA detection
10.3969/j.issn.1673-9701.2025.05.012
- VernacularTitle:基于16S rDNA分析腹泻型肠易激综合征菌群特征
- Author:
Caiyun SONG
1
;
Qiaoli LAN
1
;
Xiaoxiao LIN
1
;
Xuejian WENG
1
;
Endian ZHENG
1
Author Information
1. 温州市人民医院温州医科大学温州市第三临床学院消化内科,浙江温州 325000
- Publication Type:Journal Article
- Keywords:
Gut microbiota;
Diarrhea-predominant;
Irritable bowel syndrome;
16S ribosomal DNA gene sequencing
- From:
China Modern Doctor
2025;63(5):42-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the characteristics of intestinal flora between diarrhea-predominant irritable bowel syndrome(IBS-D)and healthy population,and to provide a clinical basis for the basic research of IBS-D.Methods A total of 32 IBS-D patients admitted to Wenzhou People's Hospital,the Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University from January 2023 to June 2024 were selected as IBS-D group,and 33 healthy during the same period as control group.Cluster diversity was determined by 16S rDNA of clusters in feces and quantification of DNA.Results The diversity of IBS-D group decreased significantly than control group,and the relative abundance of proteobacteria increased in the IBS-D group,and the relative abundance of E.coli-Shigella and Klebsiella increased in the IBS-D group.Conclusion Intestinal microbiota diversity in IBS-D patients reduced and proteobacteria is the potential pathogen of IBS-D.
