Effect of hydroxyapatite and wedelolactone on osteogenic differentiation
10.3969/j.issn.1673-9701.2025.05.001
- VernacularTitle:羟基磷灰石-蟛蜞菊内酯联合促成骨分化作用
- Author:
Di ZHU
1
;
Guangming CHEN
;
Yanqiu LIU
Author Information
1. 浙江大学医学院附属金华医院全科医学科,浙江金华 321000
- Publication Type:Journal Article
- Keywords:
Hydroxyapatite;
Wedelolactone;
Osteogenic differentiation;
Osteoporosis
- From:
China Modern Doctor
2025;63(5):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro drug release behavior of hydroxyapatite-wedelolactone(Hap-Wed)and its effect on promoting osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods The in vitro release behavior of Hap-Wed fusion materials was detected by chromatographic technique.After the cells were passed continuously up to the third generation,they were divided into:① control group:complete medium;② Os group:osteogenic inducer(1mmol/L dexamethasone,10mmol/L vitamin C,1mol/L β-glycerol phosphate);③ Wed group:1μg/ml Web and osteogenic inducer were added to the complete medium;④ Hap-Web group:1 μg/ml Web,osteogenic inducer and Hap were added to the complete medium.Alkaline phosphates(ALP)activity assay,alizarin red S staining assay,real-time quantitative polymerase chain reaction(RT-qPCR)detection of osteocalcin,Osterix and Runx2 gene expression,analysis of Hap-Wed on BMSCs osteoblast differentiation.Results Hap-Wed fusion material released the drug slowly.Compared with control group,the number of ALP staining and the number of calcified nodules increased significantly in Hap-Wed experimental group.The Hap-Wed experimental group increased gene expression of osteocalcin,Osterix and Runx2.Conclusion Hap-Wed fusion material can slow and effective stimulation of osteoblast differentiation,which may provide a promising application prospect for the future development of the carrier material.
