Study on the Repair Effects of She Ethnic Minority Medicine Primpinellae Diversifoliae DC.Extracts on Skin Photodamage by Inhibiting ROS
10.16466/j.issn1005-5509.2025.06.003
- VernacularTitle:畲药三脚风炉抑制ROS修复皮肤光损伤作用研究
- Author:
Jiancong HONG
1
;
Linghong XU
;
Huiqing LYU
Author Information
1. 浙江中医药大学药学院 杭州 311402
- Publication Type:Journal Article
- Keywords:
She ethnic minority medicine;
Primpinellae diversifoliae DC.;
reactive oxygen species;
anti-inflammation;
in vivo and in vitro experiments;
skin;
photodamage;
repair effect
- From:
Journal of Zhejiang Chinese Medical University
2025;49(6):684-689
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the restorative effects of She ethnic minority medicine Primpinellae diversifoliae DC.extracts(PEs),on skin photodamage through animal models of skin photodamage and in vitro experiments with human immortalized keratinocytes(HaCaT)cells,providing a scientific basis for clinical applications.[Methods]Ultraviolet(UV)radiation-induced skin photodamage model in female ICR mice was established,and the effects of topical application of PEs on histopathological changes in mouse skin tissue were observed using hematoxylin-eosin(HE)staining.In the photodamage model of HaCaT cells induced by H2O2,cell viability was assessed through 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay,the levels of reactive oxygen species(ROS)were measured with ROS assay kit.And the expression levels of inflammatory targets interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in skin tissue of photodamaged mice model and HaCaT cells were detected with quantitative real-time polymerase chain reaction(qPCR).[Results]PEs significantly alleviated the thickening of the epidermis and dermis induced by UV in mice(P<0.001),and significantly inhibited the mRNA expression levels of TNF-α,IL-1β and IL-6 in the skin(P<0.05,P<0.01,P<0.001).In vitro,PEs significantly reduced the ROS levels in HaCaT cells induced by H2O2(P<0.001),and significantly inhibited the mRNA expression of IL-1β,TNF-α and IL-6(P<0.01,P<0.01,P<0.001).[Conclusion]PEs significantly alleviate UV-induced skin damage and H2O2-induced cellular damage by inhibiting oxidative stress and inflammatory responses,demonstrating great potential for repairing photodamage and warranting further development and application..
