Development and application of one kind of anaerobic bacteria sampling tube with self-generated carbon dioxide
10.3969/j.issn.1672-8270.2025.04.030
- VernacularTitle:一种自生成二氧化碳厌氧菌采样管的研制与应用
- Author:
Min FU
1
;
Bohui LI
;
Huannan YU
;
Baoguang WANG
Author Information
1. 大庆市第四医院检验科 大庆 163712
- Publication Type:Journal Article
- Keywords:
Carbon dioxide;
Anaerobic bacteria;
Indicator;
Sampling pipe
- From:
China Medical Equipment
2025;22(4):161-164
- CountryChina
- Language:Chinese
-
Abstract:
In order to facilitate the collection for anaerobic bacteria in the clinical work,one kind of sampling tube that can automatically generate carbon dioxide(CO2)was developed.According to the chemical reaction principle of acid and base,the calcium carbonate(CaCO3)and dilute hydrochloric acid(HCl)were used as reactant to produce CO2.The color stripe of CO2 concentration,which was synthesized by Nile blue A and phenolphthalein of indicators,was used to collect clinical anaerobic bacteria.The sampling tube adopted quantitative deposition of chemical reagent in each room,and autonomous control for reaction process,which can arbitrarily set CO2 concentration according to the demand of sampling,and can conduct collection in meeting the demands of multiple anaerobic bacteria for different concentrations of CO2.The sampling tube can realize anaerobic state of entire process from sampling to experiment room for anaerobic bacteria,which is benefit to increase accuracy of culturing bacteria.Its design is compact and flexible,which can be directly stored in the ward to provide use for medical staffs at any time.It has a series of advantages include reasonable design,simple operation,flexible use,high safety and non-risk of cross infection.It is suitable to be promoted in the department of laboratory of hospital at all grades.
