Proteomic analysis of serum proteins in mice with carbapenem-resistant hypervirulent Klebsiella pneumoniae bloodstream infection
10.11816/cn.ni.2025-248206
- VernacularTitle:耐碳青霉烯高毒力肺炎克雷伯菌血流感染小鼠血清蛋白组学分析
- Author:
Jinfeng BAO
1
;
Yating MA
;
Yin XU
;
Yanjuan HE
;
Yuyue WANG
;
Luoluo SHI
;
Chengbin WANG
Author Information
1. 常州市第一人民医院检验科,江苏常州 213003;解放军总医院第一医学中心检验科,北京 100853
- Publication Type:Journal Article
- Keywords:
Carbapenem resistance;
Hypervirulent Klebsiella pneumoniae;
Liquid chromatogram-mass spectrome-ter;
Bloodstream infection;
Serum amyloid A protein;
Kallikrein B1
- From:
Chinese Journal of Nosocomiology
2025;35(6):823-828
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the changes of serum proteins in the mice with carbapenem-resistant hyperviru-lent Klebsiella pneumoniae(CR-hvKP)bloodstream infection by using liquid chromatogram-mass spectrometer(LC-MS),find out the differentially expressed proteins and carry out corresponding biological function analysis.METHODS The models of ICR mice with CR-hvKP and classical Klebsiella pneumoniae(cKP)bloodstream infec-tions were established,the serum specimens were collected from the mice at 12 hours of establishment of the in-fection models and were detected by using LC-MS.The result of LC-MS was identified by using Maxquant soft-ware.The corresponding bioinformatics analysis was performed for the differentially expressed proteins.RESULTS As compared with the normal control group,there were 24 upregulated proteins and 20 downregulated proteins in CR-hvKP group.As compared with cKP group,there were 107 upregulated proteins in the CR-hvKP group.The 134 differentially expressed proteins were retrieved one by one from Uniprot database,it was found that the pro-teins were involved in biological regulation,immune process,biological interaction,movement,metabolic process,response to stimulation,signal transduction,inflammatory response,oxidative stress and angiogenesis.The signal pathway analysis involved multiple metabolism-related pathways,including complement and coagula-tion cascades,cholesterol metabolism,bacterial infection,heme clearance,and assembly,remodeling and clear-ance of plasma lipoprotein.In terms of the proteins being attached great importance,the expression levels of kal-likrein B1(KLKB1)and serpin family A(Serpina)1b were downregulated as compared with the normal control group and were upregulated as compared with the cKP group;the expression levels of serum amyloid A protein(SAA)1,SAA2,Vanin-3(VNN3)and hemoglobinβpolypeptide chain b2(HBB-b2)were upregulated as com-pared with both the cKP group and the normal control group.CONCLUSIONS The CR-hvKP bloodstream infection involves the activation and action of multiple proteins.The rise of SAA1,SAA2,VNN3 and HBB-b2 or the de-cline of KLKB1 and Serpina1b may indicate the CR-hvKP bloodstream infection.
