The effects of pulsed electric field combined gemcitabine therapy on the proliferation and stemness of HCCC-9810 cholangiocarcinoma stem cells
10.3760/cma.j.cn113884-20241231-00398
- VernacularTitle:脉冲电场与吉西他滨联用对胆管癌干细胞杀伤作用和干性抑制作用的研究
- Author:
Yingxue WANG
1
;
Jiayi DU
;
Jinshuang ZHU
;
Kunyan LI
;
Han WANG
;
Zi'ang LI
;
Jiayi GAO
;
Junyao FENG
;
Yi LYU
;
Xue CHEN
Author Information
1. 西安交通大学第一附属医院MED-X研究院-再生与重建医学研究所，西安 710049
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
Neoplastic stem cells;
Pulsed electric field;
Gemcitabine;
Cancer stemness
- From:
Chinese Journal of Hepatobiliary Surgery
2025;31(7):540-546
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of pulsed electric field (PEF) combined with gemcitabine (GEM) on the viability and stemness of HCCC-9810 cholangiocarcinoma stem cells.Methods:HCCC-9810 cholangiocarcinoma stem cells were established in serum-free, cytokine-rich medium and divided into four groups: the control group, GEM group, PEF group, and the pulsed electric field combined with gemcitabine (PEF+ GEM) group. Cell proliferation was detected using the Cell Counting Kit-8 (CCK8) assay. Cell viability and apoptosis rate were measured by flow cytometry. Cell invasion ability was assessed using the Transwell assay. The expression of stemness marker proteins CD133 and Octamer-binding transcription factor 4 (OCT4), as well as the expression of β-catenin, was detected by Western blotting.Results:Regarding cell viability, the GEM, PEF, and PEF+ GEM groups showed significantly lower cell viability and higher apoptosis rate than the control group at 24 h, 48 h, and 72 h (all P<0.05). At 48 h and 72 h, the PEF+ GEM group showed significantly lower cell viability (7.2%±0.3% and 5.9%±0.8%, respectively) than the GEM group (50.7%±0.6% and 31.0%±1.2%, respectively) and the PEF group (12.2%±0.2% and 12.8%±0.2%, respectively) (all P<0.05). Regarding stemness inhibition, the PEF+ GEM groups showed significantly lower expression levels of CD133 and OCT4 at 24 h, 48 h, and 72 h compared with the control group (all P<0.05). Notably, at 48 h, the PEF+ GEM group showed a significantly lower expression level of the OCT4 (0.61±0.02) than the GEM group (0.87±0.08) and the PEF group (1.00±0.10) ( P<0.01). Furthermore, at 24 h and 48 h, the GEM, PEF, and PEF+ GEM groups showed significantly lower expression levels of β-catenin compared with the control group (all P<0.05). Conclusion:Pulsed electric field combined with gemcitabine therapy demonstrated more effective anti-proliferation and cancer stemness inhibition effects on HCCC-9810 cholangiocarcinoma stem cells compared with either monotherapy.
