Proteomic analysis of Trichosporon asahii's response to fluconazole stress
10.11816/cn.ni.2025-240418
- VernacularTitle:阿萨希毛孢子菌响应氟康唑胁迫的蛋白质组分析
- Author:
Xin YANG
1
;
Zhikuan XIA
1
;
Junhong AO
1
;
He ZHU
1
;
Jijin LI
1
;
Jiamin WU
1
;
Lingzhi XU
1
;
Rongya YANG
1
Author Information
1. 解放军总医院第七医学中心皮肤科,北京 100010
- Publication Type:Journal Article
- Keywords:
Trichosporon asahii;
Proteomics;
Multiple reaction monitoring;
Drug stress;
Drug resistance
- From:
Chinese Journal of Nosocomiology
2025;35(6):801-806
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of fluconazole on proteomics of Trichosporon asahii so as to reveal the responding process of T.asahii to fluconazole stress and the resistance mechanisms to azoles on the protein level.METHODS T.asahii AS 2.2174 was chosen as the research subject,the minimum inhibitory concentration(MIC)of fluconazole was determined by broth microdilution assay.The protein abundance of T.asahii was detec-ted by means of tandem mass tag(TMT)technique combined with liquid chromatography-tandem mass spectrom-etry(LC-MS/MS)before and after the treatment with fluconazole(1× MIC).The differentially expressed pro-teins(DEPs)were identified based on the screening standards of fold change ≥1.20 or ≤0.83 and P＜0.05.Gene ontlogy(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis were performed for the DEPs so as to understand the biological property of the DEPs and the major biological pathways that the DEPs in-volved in.Finally,the targeted validation was carried out for the targeted differentially expressed proteins by using multiple reaction monitoring(MRM).RESULTS The MIC of fluconazole to T.asahii AS 2.2174 was 8 μg/ml.Totally 196 DEPs were identified,including 93 upregulated DEPs and 103 downregulated DEPs.The function en-richment analysis showed that the DEPs mainly participated in synthesis and metabolism of sterols,drug metabo-lism,stress response,energy metabolism and intertranslation.The targeted DEPs showed the consistent expres-sion trends in MRM target validation and TMT-LC-MS/MS.CONCLUSIONS The protein abundance of T.asahii has remarkable change under the fluconazole stress.The bioinformatics analysis reveals the complicated molecular mechanisms of T.asahii in response to the fluconazole stress,which may offer valuable ideas for understanding the drug resistance to azoles and developing new drug targets.
