Chemical constituents from Citri reticulatae Pericarpium Viride and their anti-triple negative breast cancer activities in vitro
10.3969/j.issn.1001-1528.2025.01.018
- VernacularTitle:青皮化学成分及其体外抗三阴性乳腺癌活性研究
- Author:
Xu-li DENG
1
;
Yi-yan CHEN
1
;
Hong-chuan ZHANG
1
;
Yi-mou WANG
1
;
Rui YIN
1
;
Wei-mao DONG
1
;
Zhang-xian CHEN
1
;
Kui-lin ZHU
1
;
Hong-ping HE
1
;
Fa-wu DONG
1
Author Information
1. 云南中医药大学中药学院,云南省南药可持续利用研究重点实验室,云南 昆明 650500
- Publication Type:Journal Article
- Keywords:
Citri reticulatae Pericarpium Viride;
chemical constituents;
isolation and identification;
anti-triple negative breast cancer activities;
SRB assay
- From:
Chinese Traditional Patent Medicine
2025;47(1):118-125
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Citri reticulatae Pericarpium Viride and their anti-triple negative breast cancer activities in vitro.METHODS The ethanolic extract of Citri reticulatae Pericarpium Viride was isolated and purified by silica gel,polyamide,MCI,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-triple negative breast cancer activities were screened by SRB assay,and their effects on the proliferation of triple negative breast cancer cell lines HCC1806,HCC1937 and MDA-MB-231 in vitro were evaluated.RESULTS Twenty compounds were isolated and identified as nobiletin(1),tangeritin(2),5,4'-dihydroxy-7,8-dimethoxy flavonoid(3),naringenin(4),artemetin(5),5-demethynobiletin(6),3,5,6,7,8,3',4'-pentamethoxy flavonoid(7),5,4'-dihydroxy-3,6,7,8,3'-pentamethoxyflavone(8),xanthomicrol(9),p-hydroxycinnamic acid(10),5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone(11),pectolinarigenin(12),4'-dihydroxy-5,6,7-tetramethoxyflavone(13),hispidulin(14),4',5,6,7-tetramethoxy-flavone(15),1-methyl-4-(prop-1-en-2-yl)cyclohexane-1,2-diol(16),umbelliferone(17),5-hydroxymethyl furfural(18),hydroquinone(19),1H-indole-3-carbaldehyde(20).Compound 8 showed a significant inhibitory effect with the IC50 value of(5.36±0.24)μmol/L on HCC1806 cells.CONCLUSION Compound 20 is isolated from genus Citrus for the first time,8,12-13,16-17 are isolated from this plant for the first time.Compound 8 show inhibitory effects on the proliferation of HCC1806,HCC1937 and MDA-MB-231 cells in vitro and have the strongest activities.Compounds 3-4,11-12,15,17 and 19 show strong inhibitory effect on HCC1806 cells.Compounds 15,19 also inhibit the proliferation of HCC1937 cells in vitro.
