Preparation of anisamide-modified ursolic acid self-assembled nanoparticles and their anti-drug resistance effect of enzalutamide on prostate cancer via regulation of CAFs
10.3969/j.issn.1001-1528.2025.01.005
- VernacularTitle:茴香酰胺修饰熊果酸自组装纳米粒制备及其通过调控CAFs抗恩杂鲁胺对前列腺癌耐药作用研究
- Author:
Ling-xi CHEN
1
;
Shu-ting NI
;
Wen-yang ZHAO
;
Lei CHEN
;
Kai-li HU
Author Information
1. 上海中医药大学中药学院,上海 201203;上海中医药大学交叉科学研究院,上海 201203
- Publication Type:Journal Article
- Keywords:
anisamide;
ursolic acid;
self-assembled nanoparticles;
preparation process;
enzalutamide;
cancer-associated fibroblasts(CAFs);
prostate cancer;
anti-drug resistance effect
- From:
Chinese Traditional Patent Medicine
2025;47(1):28-35
- CountryChina
- Language:Chinese
-
Abstract:
AIM To prepare anisamide-modified ursolic acid self-assembled nanoparticles,and to evaluate their anti-drug resistance effect of enzalutamide on prostate cancer.METHODS Nanoparticle precipitation method was adopted in the preparation of anisamide-modified and non-anisamide-modified self-assembled nanoparticles,respectively,after which the particle size,Zeta potential and encapsulation efficiency were determined,and the morphology was observed under transmission electron microscope.The intake of cancer-associated fibroblasts(CAFs)was investigated,after which the model for enzalutamide resistance in prostate cancer was established,CCK8 assay was applied to analyzing the sensitization effect of self-assembled nanoparticles on enzalutamide,and Western blot was used for the detection of NRG1,HER3,AKT expressions.RESULTS The anisamide-modified self-assembled nanoparticles demonstrated the average particle size,Zeta potential and encapsulation efficiency of(195.13±8.06)nm,(-29.07±0.55)mV and(94.58±0.84)％,respectively.CAFs displayed higher intake in the anisamide-modified self-assembled nanoparticles than that in the non-modified preparation and free Cy5(P＜0.05).Meanwhile,anisamide-modified self-assembled nanoparticles were able to inhibit enzalutamide resistance caused by CAFs,reduce NRG1 expression on CAFs,and anisamide-modified self-assembled nanoparticles-treated conditioned medium of CAFs could reduce HER3 and AKT expression on LNCaP cells(P＜0.05,P＜0.01).CONCLUSION Anisamide-modified ursolic acid self-assembled nanoparticles can enhance the targeting of CAFs,alleviate the drug resistance effect of enzalutamide on prostate cancer caused by CAFs,and reduce NRG1 expression in CAFs.
