Quality evaluation of"Sangdi"based on HPLC fingerprints combined with chemometrics
10.3969/j.issn.1001-1528.2025.01.003
- VernacularTitle:基于HPLC指纹图谱结合化学计量学评价"桑蒂"质量
- Author:
Ping LIU
1
;
Shi-ying LUO
;
Meng-jia LI
;
Xiao-yan TAN
;
Jian-bin SUN
;
Wei-zao LUO
;
Ce TANG
;
Yi ZHANG
Author Information
1. 成都中医药大学药学院,四川成都 611137;成都中医药大学民族医药学院,四川成都 611137
- Publication Type:Journal Article
- Keywords:
"Sangdi";
quality evaluation;
HPLC fingerprints;
cluster analysis;
principal component analysis;
orthogonal partial least squares discriminant analysis;
chemical constituents;
content determination
- From:
Chinese Traditional Patent Medicine
2025;47(1):14-21
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of Tibetan medicine"Sangdi"based on HPLC fingerprints combined with chemometrics.METHODS The analysis was performed on a 30 ℃ thermostatic Welch Ultimate AQ-C18 column(250 mm × 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2％phosphoric acid flowing at 1 mL/min in a gradient elution manner,and the detection wavelength was set at 245 nm,after which cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed,the contents of gentiopicroside,sweroside,mangiferin,isoorientin,8-hydroxy-1,3,5-trimethoxyxanthone(R2)and 1,8-dihydroxy-3,7-dimethoxyxanthone(R3)were determined.RESULTS There were 18 common peaks in the fingerprints for 15 batches of samples with the similarities of more than 0.90.Six constituents showed good linear relationships within their own ranges(R 2 ≥ 0.999 2),whose average recoveries were 96.93％-103.58％with the RSDs of 0.82％-2.9％.Various batches of samples were clustered into 2 categories,4 principal components demonstrated the accumulative variance contribution rate of 86.404％,mangiferin,gentiopicroside and isoorientin were taken as quality difference markers.CONCLUSION This stable,reliable and reproducibe method can provide a reference for the comprehensive quality evaluation of"Sangdi".
