Simultaneous content determination of nine constituents in Tongxuan Lifei Pills by QAMS
10.3969/j.issn.1001-1528.2025.01.002
- VernacularTitle:一测多评法同时测定通宣理肺丸中9种成分的含量
- Author:
Jing LIU
1
;
Wan-jun JIN
;
Zhao-hui GUO
;
Lin NI
Author Information
1. 甘肃中医药大学药学院,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
Tongxuan Lifei Pills;
chemical constituents;
content determination;
quantitative analysis of multi-components by single-marker(QAMS)
- From:
Chinese Traditional Patent Medicine
2025;47(1):7-13
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of baicalin,baicalin,hesperidin,naringin,neohesperidin,rosinic acid,glycyrrhizin,ammonium glycyrrhizate and prehumetin in Tongxuan Lifei Pills.METHODS The analysis was performed on a 30 ℃ thermostatic SVEA C18 column(4.6 mm × 250 mm,5 μm),with the mobile phase comprising of acetonitrile-water(containing 0.1％phosphoric acid)flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 250 nm.Baicalin was used as an internal standard to calculate the relative correction factors of the other eight constituents,after which the content determination was made.RESULTS Nine constituents showed good linear relationships within their own ranges(r ≥ 0.999 1),whose average recoveries were 96.15％-101.58％with the RSDs of 0.75％-1.74％.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of Tongxuan Lifei Pills.
