Establishment of HPLC characteristic chromatograms and content determination of nine constituents for Yixin Fumai Granules
10.3969/j.issn.1001-1528.2025.01.001
- VernacularTitle:益心复脉颗粒HPLC特征图谱建立及9种成分含量测定
- Author:
Xin-ru CHI
1
;
Zheng-wei CHEN
;
Jie LI
;
Ai-ying WU
;
Li-hua YIN
;
Hong-bing LIU
;
Jing-guang LU
Author Information
1. 中国海洋大学海洋药物教育部重点实验室,山东青岛 266003
- Publication Type:Journal Article
- Keywords:
Yixin Fumai Granules;
HPLC characteristic chromatograms;
chemical constituents;
content determination;
cluster analysis;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2025;47(1):1-6
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC characteristic chromatograms for Yixin Fumai Granules,and to determine the contents of sodium danshensu,protocatechualdehyde,chlorogenic acid,calycosin-7-O-β-D-glucoside,ferulic acid,rosalinic acid,salvianolic acid A,salvianolic acid B,schisandrol A.METHODS The analysis was performed on a 35 ℃ thermostatic Acutfex PA-C18 column(4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,250,280,320 nm.Subsequently,cluster analysis and principal component analysis were performed.RESULTS There were 11 characteristic peaks in the characteristic chromatograms for 15 batches of samples with the similarities of more than 0.980.Nine constituents showed good linear relationships within their own ranges(r≥0.999 6),whose average recoveries were 97.60％-107.02％with the RSDs of 0.78％-1.87％.Various batches of samples were clustered into 4 categories,2 principal components demonstrated the accumulative variance contribution rate of 89.454％.CONCLUSION This sensitive and reproducible method can provide a reference for the quality evaluation and control of Yixin Fumai Granules.
