Effect of α7nAChR agonist on intestinal injury and mitochondrial autophagy of sepsis mice
10.3969/j.issn.1673-9701.2025.02.011
- VernacularTitle:α7nAChR激动剂对脓毒症小鼠肠损伤及线粒体自噬的影响
- Author:
Liping FEI
1
;
Kankai TANG
1
;
Fengqi LIU
1
;
Zhidong CHEN
1
Author Information
1. 湖州市第一人民医院重症医学科,浙江湖州 313000
- Publication Type:Journal Article
- Keywords:
Sepsis;
Intestinal injury;
α7 Nicotinic acetylcholine receptor;
Mitochondrial autophagy
- From:
China Modern Doctor
2025;63(2):41-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of α7 nicotinic acetylcholine receptor(α7nAChR)agonist PNU-282987 on intestinal injury and mitochondrial autophagy in sepsis mice.Methods A total of 30 male C57BL/6J mice were divided into control group,sepsis group and PNU-282987 group,10 mice each group.In PNU-282987 group,1mg/kg PNU-282987 was injected intraperitoneally 1h before and 2h after modeling,respectively.Intestinal tissue were taken back 24h after modeling,and pathological changes were observed by hematoxylin-eosin staining and Chiu's score were made.Mitochondrial autophagy were observed by uranium acetate-lead citrate double staining,and α7nAChR,autophagy marker protein microtubule-associated protein 1 light chain(LC)3-Ⅱ/LC3-Ⅰ and Beclin-1,and mitochondrial autophagy-related protein PTEN-induced kinase(PINK)1 were detected by Western blot.Serum and ileum tissues were taken to detect interleukin(IL)-1β,IL-6,IL-10.Results Comparison of Beclin-1,LC3-Ⅱ/LC3-Ⅰ,PINK 1 and Parkin in the mice ileum tissues among three groups,model group were higher than control group,and PNU-282987 group were higher than model group,the difference were statistically significant(P＜0.05).Comparison of mice in Chiu's score among three groups,model group was higher than control group,and PNU-282987 group was lower than model group,the difference were significant(P＜0.05).PNU-282987 group α7nAChR level in the mice ileal tissue was higher than model group,the difference was significant(P＜0.05).Conclusion α7nAChR agonist PNU-282987 improves intestinal injury in sepsis mice,activation of mitochondrial autophagy and reduction of inflammation in ileum may be the related molecular mechanism.
