Mining mitophagy-related target genes in diabetic foot ulcers and analysing their immune relevance based on bioinformatics analysis and machine learning
10.3969/j.issn.1673-9701.2025.02.008
- VernacularTitle:基于生物信息学和机器学习挖掘糖尿病足溃疡中线粒体自噬相关靶基因并分析其免疫相关性
- Author:
Yu WU
1
;
Bin SHAN
1
;
Zhaoyang ZENG
1
Author Information
1. 甘肃中医药大学中西医结合学院,甘肃兰州 730000
- Publication Type:Journal Article
- Keywords:
Bioinformatic analysis;
Machine learning;
Diabetic foot ulcer;
Mitophagy
- From:
China Modern Doctor
2025;63(2):28-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate mitophagy in diabetic foot ulcer(DFU)target genes and analyze the immune correlation.Methods DFU related datasets were obtained from the Gene Expression Omnibus(GEO)and screened for their differential expression genes(DEGs).Mitophagy related genes(MRGs)were obtained from the Genecards database.DEGs and MRGs were intersected and analysed using gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and protein-protein interaction(PPI).Target genes were mined using four machine learning models:Support vector machine-recursive feature eliminatio,least absolute shrinkage and selection operator,random forest,extreme gradient boosting.DEGs were analysed for immune infiltration and the correlation of target genes with immune infiltration.Results GO enrichment results showed that it was mainly enriched in regulation of autophagy of mitochondrion.KEGG enrichment results showed that hypoxia inducible factor-1 signalling pathway,p53 signalling pathway and AMPK signalling pathway were mainly enriched.Machine learning results showed TP53 and CYCS as target genes.Immune infiltration results showed a clear correlation of TP53 with plasma cells,regulatory T cells,follicular helper T cells,CD8+T cells and monocytes.CYCS showed a clear correlation with B cell memory,T cells CD8+T cells,monocytes,plasma cells and regulatory T cells.Conclusion TP53,CYCS may be the target genes regulating mitophagy in DFU and have a key role in reducing the level of oxidative stress.At the same time,the dysregulation of plasma cells,T cells and other immune cells may also have some effect on mitophagy.
